以N-软酯酰基壳聚糖为壳膜的新型超声微泡造影剂的对比成像  

Ultrasound imaging of novel microbubbles contrast media with a shell of N-palmitoyl chitosan

作　　者：宾建国[1] 吴爵非[1] 黄瑞珠[1] 肖云彬[1] 李美瑜[1] 陈冬冬[1] 杨莉[2] 蒋刚彪[3] 宾建平[1] 

机构地区：[1]南方医科大学南方医院心内科,广东广州510515 [2]南方医科大学南方医院药学部,广东广州510515 [3]华南农业大学制药工程系,广东广州510642

出　　处：《中国医学影像技术》2010年第7期1197-1200,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家863计划项目(2006AA02Z478);国家自然科学基金(30870722)

摘　　要：目的制备N-软酯酰基壳聚糖微泡(PCMB)类超声造影剂,评价其超声对比成像效果。方法以N-软酯酰基壳聚糖为膜材料采用声振法制备PCMB造影剂,用光学显微镜、库尔特计数仪、Zeta电位仪检测其大小、形态、浓度和表面电位。经大鼠尾静脉团注0.1ml该造影剂观察大鼠左心室、肝脏、肾脏超声造影效果,脱机分析峰值强度(PI)、达峰时间(TTP)、峰值减半时间(HPT)、廓清时间等参数,并与全氟显进行造影性能比较。结果 PCMB分布均匀,浓度为(2.88±0.45)×109/ml,直径为(1.31±0.07)μm(99.98%微泡直径小于8μm),表面电位为(55.03±3.08)mV;对大鼠左心室、肝脏、肾脏超声造影图像清晰,PI和TTP与全氟显相近(P>0.05),但HPT及廓清时间明显延长(P<0.001)。结论以廉价和生物相容性好的N-软酯酰基壳聚糖为壳膜材料制备新型超声造影剂具有良好的超声对比成像效果。Objective To develop a novel microbubbles with N-palmitoyl chitosan （PCMB） contrast media,and to evaluate its effect on ultrasound contrast imaging. Methods N-palmitoyl chitosan solution was sonicated to create PCMB. Optical micrographs,Coulter counter and Zeta potential were used to determine the bubbles＇ size,morphology,concentration and electric potential. The contrast-enhanced images of left ventricle,liver and kidney were required during intravenous bolus-injections of 0.1 ml PCMB. ＂Quanfuxian＂ was serviced as the control. The peak intensity （PI）,time to peak intensity （TTP）,half peak intensity （HPT） and visual opacification-time were analyzed offline with PC software. Results PCMB was well-distributed with a mean concentration of （2.88±0.45）×10^9 /ml,a mean diameter of （1.31±0.07）μm （99.98% of them were smaller than 8 μm） and a surface potential of （55.03±3.08）mV. Satisfactory ultrasound contrast images of the left ventricle,liver and kidney were got after PCMB. TP and TTP of PCMB was similar to Quanfuxian （P〉0.05）,while HPT and the visual opacification-time of PCMB were obviously longer than Quanfuxian （P〈0.001）. Conclusion The novel microbubbles,with inexpensive and good-biocompatibility shell material N-palmitoyl chitosan,have excellent efficacy on ultrasound contrast imaging.

关 键 词：微泡 造影剂 软酯酰基壳聚糖 

分 类 号：R445.1[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]