葡萄根系浸提液的化感作用  

Allelopathy of grape root aqueous extracts

作　　者：李坤[1,2] 郭修武[1] 郭印山[1] 李成祥[1] 谢洪刚[2] 胡禧熙[3] 张立恒[1] 孙英妮[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161 [2]辽宁农业职业技术学院,辽宁营口115214 [3]黑龙江省农业科学院大庆分院,黑龙江大庆163316

出　　处：《应用生态学报》2010年第7期1779-1784,共6页Chinese Journal of Applied Ecology

基　　金：国家现代农业产业技术体系建设专项(nycytx-30-zp-08);辽宁省科技厅重大科技攻关项目(2008204003)资助

摘　　要：以晚红(Red Globe)葡萄组培苗为材料,研究了葡萄根系浸提液对葡萄幼苗生长的影响,并采用LC-MS技术对其中的主要化感物质进行鉴定.结果表明:0.02g.ml-1(风干根质量/浸提液体积,下同)、0.1g.ml-1、0.2g.ml-1的葡萄根系浸提液显著抑制了葡萄幼苗的生长,且浓度越高,抑制作用越强.葡萄根系浸提液中含有对羟基苯甲酸、水杨酸、苯丙酸和香豆酸4种酚酸类物质.盆栽模拟试验表明,不同浓度的(0.1、1、10mmol.L-1)水杨酸和苯丙酸显著抑制了葡萄植株的生长,随着作用浓度的升高,葡萄的株高、茎粗、地上和地下鲜质量、叶片净光合速率和根系活力降低,叶片可溶性糖、MDA含量升高,淀粉含量降低,而叶片蛋白质含量无明显变化规律.Taking the tissue-cultured seedlings of grape cultivar Red Globe as test objects,this paper examined the effects of their root aqueous extracts on seedling’s growth,with the allelochemicals identified by LC-MS.The results showed that 0.02 g·ml-1 （air-dried root mass in aqueous extracts volume;the same below）,0.1 g·ml-1,and 0.2 g·ml-1 of the aqueous extracts inhibited the growth of the seedlings significantly,and the inhibition effect increased with increasing concentration of the extracts.The identified allelochemicals of the extracts included ρ-hydroxybenzoic acid,salicylic acid,phenylpropionic acid,and coumaric acid.Pot experiment showed that different concentration （0.1,1,and 10 mmol·L-1） salicylic acid and phenylpropionic acid inhibited the seedling’s growth remarkably.With the increasing concentration of the two acids,the plant height,stem diameter,shoot-and root fresh mass,leaf net photosynthetic rate and starch content,and root activity of the seedlings decreased,while the leaf soluble sugar and MDA contents increased.No obvious change pattern was observed in leaf protein content.

关 键 词：葡萄 根系浸提液 化感作用 化感物质 

分 类 号：S663.1[农业科学—果树学]