运动疲劳对大鼠纹状体单胺类递质含量及多巴胺受体表达的影响  

Influence of exercises-induced fatigue on monoamine neurotransmitters and dopamine receptors in striatum in rats

作　　者：侯莉娟[1] 刘晓莉[1] 乔德才[1] 

机构地区：[1]北京师范大学体育与运动学院,北京100875

出　　处：《中国康复医学杂志》2010年第7期639-642,共4页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(30771050);北京市自然科学基金资助项目(5072024)

摘　　要：目的:通过测定纹状体单胺类神经递质及多巴胺受体表达量在运动疲劳前后的变化,探讨纹状体多巴胺系统信号通路在运动疲劳后中枢调控中的作用。方法:雄性SD大鼠随机分为对照组和实验组,每组12只大鼠。以10天递增负荷游泳运动建立大鼠运动疲劳模型。采用高效液相色谱法及实时荧光定量PCR技术,测定纹状体区5-羟色胺、去甲肾上腺素、5-羟基吲哚乙酸、肾上腺素、多巴胺、3,4-二羟基苯乙酸、高香草酸及多巴胺受体的表达量。结果:实验组纹状体5-羟色胺含量较对照组增加14.23%(P<0.05),多巴胺含量增加27.35%(P<0.01);运动疲劳后纹状体多巴胺Ⅰ型受体没有出现显著浓度变化,而多巴胺Ⅱ型受体浓度显著升高(P<0.01)。结论:运动疲劳使纹状体区5-羟色胺、多巴胺与多巴胺Ⅱ型受体含量增高,多巴胺可能与Ⅱ型受体结合激活间接通路影响锥体外系对运动功能的调节。Objective：To testing monoamine neurotransmitters and dopamine receptors concentration after exercises-induced fatigue and to discuss the role of striatum in central regulation of dopamine system signal path way after exercises-induced fatigue.Method：SD rats were randomly divided into experiment group and control group,12 rats in each group.Ten-day swimming exercises with progressively increasing load protocol was used to set up exercises fatigue rat model.HPLC and real-time PCR were used to determine the changes of 5-HT,NE,DA,and their metabolic products contents and dopamine receptors.Result：In experiment group the contents of 5-HT in striatum increased by 14.23% compared with that in control group（P0.05）,DA increased by 27.35%（P0.01）;D2DR increased significantly compared with that in control group（P0.01）.Conclusion：After exercises-induced fatigue,the increased dopamine maybe combine with D2DR to activate indirect pathway to regulate extrapyramidal system.

关 键 词：运动疲劳 纹状体 单胺类递质 多巴胺受体 

分 类 号：R87[医药卫生—运动医学]