Fragmentation study of two brassinolides by ion trap tandem mass spectrometry  

作　　者：HUO FeiFeng BAI Yu LIU HuWei 

机构地区：[1]The Key Lab of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

出　　处：《Chinese Science Bulletin》2010年第21期2219-2224,共6页

基　　金：supported by the National Natural Science Foundation of China(90717002 and 90717002);the Fundamental Research Funds for the Central Universities;the Project Sponsored by the Scientific Research Fundation for the Returned Overseas Chinese Scholars,Education Ministry of China

摘　　要：The fragmentation pathways of two isomers of brassinosteroids,28-homobrassinolide(28-h-BL)and 28-epihomobrassinolide(28-eh-BL),have been investigated by tandem mass spectrometry(MSn,n=1,2,3,4)with the electrospray ionization(ESI) source.The ESI mass spectrometric fragmentation pathways of protonated 28-h-BL and 28-eh-BL were comprehensively elucidated,and the principles revealed in this investigation could potentially be used to identify and distinguish brassinosteroids and their isomers.The fragmentation pathways of two isomers of brassinosteroids, 28-homobrassinolide （28-h-BL） and 28-epihomobrassinolide （28-eh-BL）, have been investigated by tandem mass spectrometry （MSn, n = 1, 2, 3, 4） with the electrospray ionization （ESI） source. The ESI mass spectrometric fragmentation pathways of protonated 28-h-BL and 28-eh-BL were comprehensively elucidated, and the principles revealed in this investigation could potentially be used to identify and distinguish brassinosteroids and their isomers.

关 键 词：高油菜素内酯 串联质谱法 离子阱 电喷雾质谱 光谱分裂 异构体 MSN ESI 

分 类 号：Q946[生物学—植物学]