黑根霉对人参皂苷Re的生物转化  

Ginsenoside Re biotransformed by Rhizopus sp.

作　　者：包海鹰[1] 李磊[1] 昝立峰[1] 王春玲[1] 苏日古格[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《菌物学报》2010年第4期548-554,共7页Mycosystema

基　　金：人参皂苷RG3;RG5;RK1益智作用机理的研究(No212-00026)

摘　　要：利用菌种黑根霉Rhizopus sp.对人参皂苷Re进行生物转化,并对人参皂苷Re及其发酵产物进行HPLC系统分析比较,经液相色谱-质谱分析得出人参皂苷Re转化率为92.16%,并制备出人参皂苷Re发酵产物中峰值升高的成分,转化后的人参皂苷发酵产物中化合物1确定为人参皂苷Rg2,化合物2为Rg2的同分异构体,得率为10.13%;化合物3和化合物4确定为人参皂苷Rg5/Rk1,得率为29.23%。从结果初步推测得出人参皂苷Re被黑根霉转化为人参皂苷Rg2的机理,人参皂苷Re转化成人参皂苷Rg5/Rk1的机理还有待于进一步研究。Ginsenoside Re was transformed by Rhizopus sp.,and Re fermentation products were compared by HPLC system analysis.Liquid chromatography（LC） and mass spectrum（MS） analysis showed the conversion ratio of ginsenoside Re was 92.16%.The raised compound 1 in the panaxatriol fermentation products was identified as ginsenoside Rg2 with yield of 10.13%,while compound 2 and compound 3 respectively as ginsenoside Rg3 and Rk1 with yield of 29.23%.The results showed that Re can be biotransformated by Rhizopus sp.It was inferred that ginsenoside Re had been biotransformed into ginsenoside Rg2.The transformation of ginsenoside Re into Rg5/Rk1 needs further studies.

关 键 词：人参皂苷RE 发酵产物 高效液相色谱 

分 类 号：S567.51[农业科学—中草药栽培]