TNF-α对放烧复合伤小鼠骨髓细胞IL-3R调节作用的研究  

Regulative effects of recombinant human tumor necrosis factor α on interleukin 3 receptor of bone marrow nucleated cells after combined radiation burn injury in mice

作　　者：黄定德[1,2] 程绍钧[1,2] 罗成基[1,2] 张广运 郭朝华[1,2] 

机构地区：[1]第三军医大学预防医学系防原医学教研室 [2]第三军医大学医学检验系核医学教研室

出　　处：《第三军医大学学报》1999年第1期34-37,共4页Journal of Third Military Medical University

摘　　要：目的：研究重组人肿瘤坏死因子α（ＴＮＦ－α）对放烧复合伤小鼠骨髓有核细胞（ＢＭＮＣ）ＩＬ－３Ｒ的调节作用。方法：以放烧复合伤小鼠为模型，观察一次性静脉注入不同剂量的ＴＮＦ－α对ＢＭＮＣ增殖的影响及１０万ＵＴＮＦ－α对ＩＬ－３Ｒα表达及功能、ＢＭＮＣ数量的调节作用。结果：①静注不同剂量的ＴＮＦ－α对放烧复合伤小鼠ＢＭＮＣ增殖的影响不一；②１０万Ｕ的ＴＮＦ－α１、３ｄ上调放烧复合伤小鼠ＩＬ－３Ｒα的平均含量（对阳性率及功能无明显影响），而致ＢＭＮＣ数量明显降低；７ｄ时恢复至对照组水平；③１０万ＵＴＮＦ－α１、３ｄ致放烧复合伤小鼠ＢＭＮＣ计数明显降低。结论：１０万ＵＴＮＦ－α在体内虽能上调放烧复合伤小鼠ＩＬ－３Ｒα的平均含量而剌激造血，但可能因其同时激活造血调控网络整体效应却抑制骨髓造血。Objective: To investigate the effects of recombinant human tumor necrosis factor α (RHTNF α) on interleukin 3 receptor (IL 3R) of bone marrow nucleated cells (BMNCs) after combined radiation burn injury in mice. Methods: After the combined radiation burn injury was inflicted on the mice, the effects of RHTNF α at different doses on proliferation of BMNCs and the regulative effects of a single injection of 10 5U of RHTNF α on the expression and function of IL 3R α and BMNC counts were determined. Results: ①Different doses of RHTNF α exerted different effects on the proliferation of BMNCs in vivo. ②The mean content of IL 3R α was up regulated on the 1st and 3rd d but recovered to the normal on the 7th d after the injection of 10 5 U of RHTNF α. ③The single injection of 10 5 U of RHTNF α caused a rapid decrease in BMNC counts on the 1st day after injection and the counts remained low up to the 3rd. Conclusion: A single injection of 10 5 U of RHTNF α can up regulate the expression of IL 3R of BMNCs after combined radiation burn injury, which stimulates hematopoiesis. However, it causes a significant decrease in BMNC counts because of the effects of the hematopoietic network.

关 键 词：肿瘤坏死因子Α 放烧复合伤 骨髓 IL-3R 

分 类 号：R818.740.6[医药卫生—放射医学] R644[医药卫生—临床医学]