利用双重PCR技术检测柑橘溃疡病菌  

Douplx PCR Method for Detecting of the Xanthomonas axonopodis pv. citri

作　　者：陈小帆[1] 莫瑾[1] 左静[1] 王象贤[1] 王光锋[1] 彭梓[1] 钟文英[1] 朱金国[1] 

机构地区：[1]湖南出入境检验检疫局,湖南长沙410004

出　　处：《华南农业大学学报》2010年第3期32-35,共4页Journal of South China Agricultural University

基　　金：国家质量监督检验检疫总局课题(2006IK155);"十一五"国家科技支撑计划项目(2006BAD08A16)

摘　　要：建立了柑橘溃疡病菌的双重PCR检测方法.根据柑橘溃疡病菌的基因片段OPM-12SCAR fragment(AF312370),设计特异性PCR检测引物,并结合rpf基因设计的引物对柑橘溃疡病菌和其近缘菌株、植物原性细菌的DNA进行了双重PCR检测方法的研究.该方法特异性强,菌液浓度检测灵敏度达到102CFU/mL,能够满足快速、准确诊断柑橘溃疡病菌的要求.A PCR-DHPLC method was developed for detecting Xanthomonas axonopodis pv. citri. It was established by using one pair of primers unique to OPM-12 SCAR fragment （GeneBank AF312370） and the other pair of primers was reported unique to rpf gene. By using the two pairs of primer,the douplx PCR could be a special method for monitor Xanthomonas axonopodis pv. citri and could detect 10^2 CFU/mL bacterial cells. The result showed approach method for detecting that the douplx PCR method would be a rapid, reliable, sensitive and simple Xanthomonas axonopodis pv. citri.

关 键 词：双重PCR 柑橘溃疡病菌 检测 

分 类 号：Q939.95[生物学—微生物学]