整体低氧预处理对大鼠肺缺血再灌注损伤的保护作用及机制  

Protective effect of whole-body hypoxic preconditioning on lung ischemic reperfusion injury in rats and its mechanism

作　　者：陆德琴[1] 李涛[2] 陈莹[3] 李保罗[1] 

机构地区：[1]贵阳医学院病理生理学教研室,贵阳550004 [2]梅州市嘉应学院医学院 [3]贵阳护理职业学院

出　　处：《山东医药》2010年第27期15-17,共3页Shandong Medical Journal

基　　金：贵州省优秀科技教育人才省长专项基金资助项目(黔科教办(2003)04号)

摘　　要：目的探讨整体低氧预处理(WHPC)对肺缺血再灌注(I/R)损伤的保护作用及可能机制。方法将50只肺I/R损伤模型大鼠随机分为5组。模型组不干预;制模前30 min WHPC组行WHPC;5-羟癸酸(5-HD)+WH-PC组制模前30 min静注5-HD 10 mg/kg、行WHPC;二氮嗪(DE)组制模前30 min腹腔注入DE 10 mg/kg;5-HD+DE组制模前30 min静注5-HD 10 mg/kg,15 min后腹腔注射DE 10 mg/kg。观察各组肺组织病理形态学变化、肺湿/干重比变化,采用分光光度计比色法检测肺组织丙二醛(MDA)含量及髓过氧化物酶(MPO)活性,采用TUNEL法检测肺组织细胞凋亡指数(AI)变化。与对照组(假手术,不行干预)比较。结果与对照组比较,模型组肺组织出现明显损伤性形态学改变,肺湿/干重明显增加,肺组织MDA含量和MPO活性明显增高,AI亦明显增高。与模型组比较,WHPC组和DE组肺组织损伤性病理改变明显减轻,肺湿/干重明显降低,MDA含量、MPO活性及AI亦均明显降低。结论 WHPC对大鼠肺缺血再灌注损伤有明显保护作用,其机制可能为WHPC促使线粒体ATP敏感钾通道开放。Objective To investigate the protective effect of whole-body hypoxic preconditioning（WHPC） on lung ischemia-reperfusion（I/R） induced injury in rats and its probable mechanism.Methods Fifty lung I/R injury rats were randomly divided into 5 groups.The model group was not given any intervention;the WHPC group was given WHPC 30 mins before the model preparation;the 5-hydroxydecanoic acid（5-HD） ＋WHPC group was injected 5-HD 10 mg/kg and given WHPC 30 mins before the model preparation;the Diazoxide（DE） group were preconditioned with DE（10 mg/kg） intraperitoneally 30 mins before the model preparation;5-HD＋ DE group received 5-HD 10 mg/kg）intravenously injection 30 mins before the model preparation and intraperitoneally injection of DE 10 mg/kg 15 mins after that.Morphological changes and the wet-to-dry weight ratio of lung tissue in each group were detected.The content of malondialdehyde（MDA） and myeloperoxidase（MPO） in lung tissue were detected with spectrophotometer.TUNEL was used to determine apoptosis index.Results Compared with the control group,morphologic injury in model group was severely,the wet-to-dry ratio of lung tissue increased significantly,the content of MDA and MPO activity in lung tissue enhanced,and the apoptosis index dramatically increased.Compared with the model group,the lung tissue damage of the WHPC group and the DE group reduced obviously,the wet-to-dry ratio decreased obviously,the MDA content,MPO activity and AI also reduced significantly.Conclusion WHPC can protect the lung I/R injury in rats,which may be mediated by opening of mitochondrial ATP sensitive potassium channel.

关 键 词：再灌注损伤 低氧预处理 线粒体ATP敏感钾通道 

分 类 号：R563.9[医药卫生—呼吸系统]