白细胞介素-4基因多态性与儿童哮喘易感性的关系  

Association between genetic polymorphism in interleukin-4 and the risk of childhood asthma

作　　者：张林媛[1] 冷曙光[1] 张亚玮 钮式如[3] 陆宝玉[3] 戴敏[4] 金银龙[3] 郑同章 郑玉新[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [2]Department of Epidemiology and Public Health,Yale University School of Public Health [3]中国疾病预防控制中心环境与健康相关产品安全所 [4]中国医学科学院肿瘤医院肿瘤研究所

出　　处：《毒理学杂志》2010年第3期175-178,179,共5页Journal of Toxicology

基　　金：美国国立卫生研究院(NIH)Fogarty培训基金(1D43TW008323-01;1D43TW007864-01);国家自然科学基金(30625031)

摘　　要：目的探讨白细胞介素-4(IL-4)基因多态性与儿童哮喘患病风险的关系。方法采用以人群基础的病例对照研究(293例哮喘儿童和668例对照儿童)方法 ,进行IL-4C-590T基因多态与儿童哮喘风险关联分析,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)进行基因分型。结果未发现IL-4C-590T多态性与儿童哮喘显著关联,但按照性别分层后,在女童中CC基因型携带者哮喘患病危险性显著降低,OR值为0.10(95%CI:0.01～0.77)。IL-4-590CC和CT基因型与饲养鸽子或其他鸟类两因素共同存在时,有明显的正向交互作用,产生交互作用的ORint值为2.562(P=0.008),哮喘患病风险中可以归因于两因素交互作用的比例(AP)占63%,交互作用指数(S)达到1.392。IL-4-590CC和CT基因型与被动吸烟也存在正向交互作用,ORint值为1.836(P=0.064),达到临界显著性。结论在女童中发现IL-4C-590T多态性与儿童哮喘患病风险显著关联,并且该基因变异与饲养鸽子或其他鸟类、被动吸烟存在正向交互作用。Objective To investigate association of genetic polymorphism of interleukin-4 with the risk of childhood asthma. Methods A population-based case-control study of 293 cases and 668 controls was conducted to investigate the association between polymorphism in IL-4 and susceptibility to childhood asthma. Genotypes of IL-4 C-590T were analyzed by polymerase chain reaction-restriction fragment length polymorphism method. Results No association was found between IL-4 C-590T polymorphism and the risk of childhood asthma in study subjects. After stratified by gender,the risk of asthma for the girls with genotype CC was significantly decreased,OR = 0. 10（95% CI： 0. 01 ～ 0. 77）. A significant gene-environment interaction was observed between C-590T polymorphism and pigeon or birds in house,with OR of interaction 2. 562（P = 0. 008）,AP 63% , and index S 1. 392. A marginal significant gene-environment interaction was also observed between C-590T polymorphism and passive smoking,of which ORintwas 1. 836（P = 0. 064）. Conclusion The association between polymorphism of IL-4 C-590T and childhood asthma was found among girls,and positive interaction existed between polymorphism of IL-4 and pigeon or birds in house or passive smoking.

关 键 词：儿童哮喘 白细胞介素 遗传易感性 基因-环境交互作用 

分 类 号：R135[医药卫生—劳动卫生]