染色体分离技术及其在植物中应用研究进展  

Research Advances of Chromosome Micro-dissection Technology and Its Utilization in Plants

作　　者：刘玉玲[1,2] 王坤波[1] 

机构地区：[1]中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南安阳455000 [2]安阳九州药业有限责任公司,河南安阳455000

出　　处：《安徽农业科学》2010年第16期8321-8324,共4页Journal of Anhui Agricultural Sciences

基　　金：中央级公益性院所基本科研业务费资助项目(SJA0901);国家863计划项目(2009AA101104);国家自然科学基金项目(30170501)

摘　　要：染色体分离技术是分子遗传学和细胞遗传学的桥梁技术,得以在诸多领域广泛应用。就染色体分离技术的原理、主要流程及存在的主要问题进行了总结,同时对该技术在植物中应用研究进展进行了阐述。Chromosome micro-dissection technique is a direct bridge from cytogenetics research to molecular genetics research.It has been widely used in many fields.The principle of chromosome micro-dissection technique and the major procedures were reviewed.And some major technical problems such as chromosome identification,micro-dissection and micro-cloning were discussed and the application progress of this technique in plants was illustrated.

关 键 词：染色体 显微分离 微克隆 植物 

分 类 号：Q813.4[生物学—生物工程]