鞘内注射抗生长抑素血清对佐剂性关节炎大鼠电针镇痛及中枢β-EP水平的影响  

EFFECTS OF INTRATHECAL INJECTION OF ANTI-SOMATOSTATIN SERUM ON ELECTRIC ACUPUNCTURE ANALGESIA AND β-ENDORPHINS IN RATS WITH ADJUVANT-INDUCED ARTHRITIS

作　　者：张皓[1] 王健[1] 陈新勇[1] 吴富东[1] 单秋华[2] 

机构地区：[1]山东中医药大学,山东济南250014 [2]山东中医药大学附属医院

出　　处：《齐鲁医学杂志》2010年第5期402-404,共3页Medical Journal of Qilu

基　　金：国家自然基金资助项目(30772829)

摘　　要：目的观察鞘内注射抗生长抑素血清(ASSS)对佐剂性关节炎(AA)大鼠痛阈、电针(EA)镇痛及中枢β-内啡肽(-βEP)水平的影响,探讨其可能的镇痛机制。方法以AA大鼠为炎症痛模型,以痛阈为指标观察鞘内注射ASSS对痛阈和EA镇痛的影响;以放免法测定AA大鼠下丘脑、脊髓中-βEP含量,观察鞘内注射ASSS及EA镇痛对其影响。结果鞘内注射ASSS可显著降低AA大鼠痛阈,并使EA镇痛效应明显降低;EA镇痛可提高下丘脑和脊髓-βEP含量,鞘内注射ASSS可使中枢-βEP水平进一步显著升高。结论内源性生长抑素在痛觉调制中起重要作用,有显著的镇痛效果并与增强EA镇痛作用密切相关;其镇痛机制可能与中枢-βEP水平相关。Objective To observe the effects of intrathecal injection of anti-somatostatin serum（ASSS） on electric acupuncture（EA） analgesia and β-EP in rats with adjuvant-induced arthritis（AA）,and explore potential mechanism of analgesia.Methods AA rats were taken as a model of pain caused by inflammation,and pain threshold as the index,the effect of intrathecal injection of ASSS on pain threshold and EA was observed;the levels of β-EP in spinal cord and hypothalamus were detected byusing radioimmunoassay. Results Intrathecal ASSS remarkably reduced the threshold of pain,lower the analgesic effects of EA.EA increased the β-EP level in spinal cord and hypothalamus,which further raised the levels of β-EP in central nervous system.Conclusion Endogenous somatostatin plays an important role in analgesic modulation with notable effect of pain relief and closely associated with enhancement of EA,the mechanism of analgesia might be related to β-EP level of central nervous system.

关 键 词：电针 镇痛 关节炎 实验性 生长抑素 Β内啡肽 

分 类 号：R245.3[医药卫生—针灸推拿学]