葡萄叶片蛋白质双向电泳技术体系的建立  

Study on the Conditions of 2-DE Analysis of Proteome in Grape Leaveas

作　　者：张玉洁[1] 张军科[1] 罗世杏[1] 

机构地区：[1]西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌712100

出　　处：《北方园艺》2010年第13期147-151,共5页Northern Horticulture

基　　金：陕西省重大攻关资助项目(2006KZ05-G5)

摘　　要：现对葡萄叶片中总蛋白质的双向电泳技术体系进行了初步探讨,比较了不同等电聚集程序、不同pH胶条以及不同SDS-PAGE凝胶浓度的双向电泳效果。结果表明：葡萄叶片蛋白质使用等电聚焦程序B、pH范围为4~7的胶条和12%的SDS-PAGE凝胶可以得到较为理想的双向电泳分析结果;采用7 cm胶条最大可以分离出143个有效蛋白质点。The method system of 2-dimensional electrophoresis（2-DE）of protein in grape leaves was studied,the results of 2-dimensional electrophoresis from different procedures of isoelectric focusing,different immobilized pH gradients strips and with different SDS-PAGE gel concentration were compared.The results showed that isoelectric focusing procedure B,immobilized pH gradients（IPG）strip with the linear range of pH 4~7 and 12% SDS-PAGE gel were the better choice,more than 143 protein spots were separated in one run.

关 键 词：葡萄 蛋白质组 双向电泳 

分 类 号：S663.1[农业科学—果树学]