Prd29A:PaDREB2植物表达载体的构建及转化新疆杨的研究  

Study on Construction of Plant Expression Vector Carrying Prd29A:PaDREB2 Gene and Its Genetic Transformation in Populus bolleana

作　　者：秦红霞[1] 张海超[2] 刘敬梅 宋玉霞[4] 

机构地区：[1]九江学院生命科学学院,江西九江750021 [2]中国药科大学遗传育种教研室,江苏南京210038 [3]北京未名凯拓农业生物技术有限公司,北京100085 [4]宁夏农业生物技术重点实验室,宁夏银川750002

出　　处：《北方园艺》2010年第13期160-163,共4页Northern Horticulture

基　　金：九江学院科研资助项目(07kj9)

摘　　要：PaDREB2是从银新杨(Populus alba×P.albavar.pyramidalis)中克隆得到的DREB类转录因子,该研究将逆境诱导型rd29A基因的启动子和PaDREB2构建到植物表达载体pCAMBIA1301上,并以新疆杨(Populus albavar.pyramidalis)试管苗的叶片外植体为受体,利用根癌农杆菌介导的方法进行遗传转化。筛选得到110株潮霉素抗性植株,经PCR检测,其中25株抗性植株存在目的基因Prd29A:PaDREB2。PaDREB2,a DREB-like transcription factor,was cloned from Yinxin populus（P.alba×P.alba var.pyramidalis）.In the study,stress inducible gene promoter Prd29A and PaDREB2 were builted into the plant expression vector pCAMBIA1301.And we have transformed the explants of Populus bolleana leaves by Agrobacterium-mediated genetic transformation system.A total 110 resistant plants were gained via strictly selection by hygromycin.Among them 25 positive transgenic plants were verified by PCR analysis.

关 键 词：新疆杨 Prd29A:PaDREB2基因 遗传转化 植物表达载体 

分 类 号：Q943.2[生物学—植物学] S792.11[农业科学—林木遗传育种]