花粉管通道导入外源DNA方法的研究  

Study on Techniques of Introduction of Exogenous DNA into Plants via Pollen Tube Pathway

作　　者：陈彦[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《北方园艺》2010年第13期226-228,共3页Northern Horticulture

基　　金：山东省自然科学基金资助项目(Y2008D41)

摘　　要：在总结前人研究的基础上,认为花粉管通道法导入外源DNA的方法可分为花粉粒携带法、子房注射法和柱头切除法。3种导入外源基因的方法已在植物转基因育种研究中被广泛运用。在实际运用中应针对某一特定的受体植物,根据受体植物的雌蕊结构特点选择合适的导入外源基因的方法,可提高花粉管通道法的转化效果和转化率。The pollen-mediated introduction,which introduced exogenous DNA to acceptors by the treatment of the pollen grains or the stigma of acceptor with the donor DNA or the donor pollen grains,was studied earlier.The stigma excision-mediated introduction was a method of addition of exogenous DNA to the excided style of the acceptor plants.The direct injection of exogenous DNA into ovary of acceptor via a microinjector was called ovary injection-mediated introduction,also called ovary microinjection-mediated introduction.All these three method of introduction of exogenous DNA into acceptors have been universal in plant genetic transformation.An optimal method obtained by studying the structure of the pistil of the acceptor could make higher transgenic transformation rate and more efficient transformations.

关 键 词：花粉管通道法 外源DNA 花粉粒携带法 柱头切除法 子房注射法 

分 类 号：Q943.2[生物学—植物学]