铜对中华鲟幼鱼的急性毒性及对肝脏抗氧化酶活性的影响  

Effects of antioxidant enzyme in liver and acute toxicity of Cu^(2+) on juvenile Chinese sturgeon

作　　者：姚志峰[1,2] 章龙珍[1,2] 庄平[1,2] 黄晓荣[1,2] 颜世伟[3] 

机构地区：[1]中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源及生态重点开放实验室,上海200090 [2]上海海洋大学水产与生命学院,上海201306 [3]大连水产学院生命科学与技术学院,辽宁大连116023

出　　处：《中国水产科学》2010年第4期731-738,共8页Journal of Fishery Sciences of China

基　　金：国家自然科学基金重大项目(30490234);上海市科委研究计划项目(08391910300);科技部科技基础工作和社会公益研究专项(2003DIB4J129);上海市长江口中华鲟自然保护区专项资助项目;中央级公益性科研院所基本科研业务费专项资金项目(2007M023;2008M01)

摘　　要：采用静水生物测试法研究铜(Cu2+)对中华鲟(Acipenser sinensis Gray)幼鱼的急性毒性并进行安全评价;根据预实验结果,设定0.005mg/L、0.01mg/L和0.015mg/L3个Cu2+浓度梯度进行急性暴露实验,以肝脏组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽抗氧化酶(GSH-PX)活性为指标研究Cu2+污染对中华鲟幼鱼的毒理作用。结果表明,Cu2+浓度升高对中华鲟幼鱼产生了较大的毒性,24h、48h、72h和96h LC50分别为0.05932mg/L、0.0340mg/L、0.0283mg/L和0.0217mg/L,安全质量浓度为0.00217mg/L;不同浓度Cu2+暴露时,中华鲟幼鱼肝组织中SOD、CAT、GSH-PX的活性在暴露24h时被显著抑制(P<0.05),在处理48h后酶活性逐渐恢复,随后又逐渐下降,其下降幅度与Cu2+质量浓度呈正相关(P<0.05)。研究认为,SOD、CAT和GSH-PX活性变化可以反映中华鲟幼鱼受伤害的程度,并可用做中华鲟安全性风险评价的参考依据。The acute toxicity of Cu2＋ and the changes of antioxidant enzyme of Chinese sturgeon（Acipenser sinensis Gray）were studied by the static test method. According to this LC50,the experiment concentrations of CuSO4 were set up at 0,0.005 mg/L,0.01 mg/L and 0.015 mg/L and the exposure time were set up at 24 h,48 h,72 h and 96 h. The results showed that copper was a drastic toxicant;the 24 h,48 h,72 h and 96 h median lethal concentrations of copper were 0.0593 2 mg/L,0.034 0 mg/L,0.028 3 mg/L and 0.021 7 mg/L respectively,the safe concentration of these metal was 0.002 17 mg/L. The activities of SOD,CAT and GSH-PX in the liver tissues of Chinese Sturgeon were restrained obviously within 24 h,but the activities recovered gradually from24 to 48 h,the activities decreased after 48 h,and it was lower than that of the contro1 group. The changes of the activities of SOD,CAT and GSH-PX indicated the damage degree of Chinese Sturgeon by Cu2＋.

关 键 词：中华鲟幼鱼 铜 安全浓度 肝脏 抗氧化酶 

分 类 号：S94[农业科学—水产养殖]