马动脉炎病毒GP5蛋白主要抗原域的表达及鉴定  

Prokaryotic expression and identification of equine arteritis virus GP5 protein major antigenicity domain

作　　者：侯玉杰[1,2] 时成龙[1,2] 相文华[2] 郭巍[2] 李红梅[2] 赵立平[2] 胡兰[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110161 [2]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国兽医杂志》2010年第6期18-21,共4页Chinese Journal of Veterinary Medicine

摘　　要：在分析EAV-GP5蛋白抗原性的基础上,设计一对引物扩增GP5蛋白的主要抗原域编码EAV-GP5 79~330 bp。将扩增基因插入pET-30aBamHⅠ和HindⅢ间构建原核表达载体pET-GP5。将pET-GP5质粒转入BL21（DE3）宿主菌并优化诱导表达条件,实现马动脉炎病毒EAV-GP5蛋白主要抗原域的高效表达。重组pGP5经纯化制备兔抗pGP5免疫血清,经免疫印迹及间接免疫荧光试验鉴定,证实所得表达产物具有良好的免疫原性。Based on EAV GP5 gene seguence a pair of primers were designed to amplify gene fragment encoding EAV GP5.The amplified gene was inserted into pET-30a between BamHI and HindⅢ.The prokaryotic expression vector pET-gGP5 was constructed and transformed into BL21（DE3） host bacteria.The recominbinant protein was purified and used to prepare high-titep-immuno-serum of Changbai rabbits.Western-blot result indicated that the purified protein product has a good antigenicity

关 键 词：马动脉炎病毒 GP5蛋白 免疫原性 

分 类 号：S852.659.1[农业科学—基础兽医学]