UPLC法进行柴胡疏肝散体内外成分的同步定性测定  

Simultaneously Qualitative Determination of Multiple Compounds in Chaihu Shugan san(CSS) and in Rat Intestine by UPLC-PDA

作　　者：胡思航[1,2,3] 黄熙[1,2,3] 梁清华 秦锋[1,2,3] 邱新建[1,2,3] 任平 范荣 唐涛 张春虎 

机构地区：[1]中南大学湘雅医院中药药理实验室,长沙410008 [2]国家中医药管理局肝脏象重点研究室,长沙410008 [3]国家中医药管理局"十一五"计划脑病重点专科建设,长沙410008

出　　处：《医学研究杂志》2010年第7期42-45,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(90409010;30572339)

摘　　要：目的进行柴胡疏肝散汤剂中多成分及其灌胃该方后大鼠小肠中吸收成分的同步定性测定。方法建立UPLC-PDA测定的新方法,同步定性测定:柴胡疏肝散汤剂中的11种成分;灌胃(35g/kg)该方30min后健康大鼠小肠中的吸收成分;色谱条件为ACQUITY UPLC BEH C18柱(2.1mm×50mm,1.7μm),流动相为乙腈-0.5%醋酸水,流量:0.5ml/min,温度:40℃,检测波长:190～480nm。结果在41min内,该方中阿魏酸、芍药苷、柚皮苷、橙皮苷、水合橙皮内酯、新橙皮苷、异甘草素、甘草酸铵、芍药内酯苷、甘草苷和甘草次酸12种成分及灌胃该方30min后健康大鼠小肠中的上述前8种成分获得良好分离,各成分的LOD在0.166×10-3～1.44μl/ml,信噪比(S/N)≥3。结论该方法快速、灵敏;上述的结果为该方进行质量控制及其药动学研究奠定了方法学基础。ObjectiveTo simultaneously qualitative determinate the multi-component in Chaihushugansan（CSS） and in intestine in rat following oral CSS. MethodsA novel UPLC-PDA method was developed to qualitate simultaneously 11 compositions in CSS and absorbed components in intestine 30 min after oral CSS in healthy SD rats （35g/kg）. The chromatographic conditions： analytical column was a Waters BEH （R） C18 Column （2.1mm× 50mm i.d., particle size 1.7μm）. The mobile phase contained A-acetonitrile and B-0.5% acetic acid. The flow rate was 0.5ml/min, and the column temperature was maintained at 40℃. The detection wavelengths were between 190~480nm. ResultsFerulic acid, paeoniflorin, naringin, hesperidin, merazin hydrate, neohesperidin, isoliquiritigenin, glycyrrhizic acid, albiflorin, liquiritin and 18-bata-glycyrrhizic acid in CSS and the former 8 compounds in the rat intestine were well separated within 41min. LODs of component （μl/ml） were 0.166×10-3~1.44 （S/N≥3）. ConclusionThe method is rapid and sensitive, which helps to further develop the quantitative method of above compounds for CSS’s quality control and pharmacokinetics.

关 键 词：柴胡疏肝散 UPLC-PDA SD大鼠 小肠 定性测定 

分 类 号：R289.5[医药卫生—方剂学]