虎杖苷吸入剂对哮喘大鼠肺组织TNF-α和Eotaxin基因表达的影响  

Effects of Polydatin Inhalation on the TNF-α and Eotaxin Gene Expression in the Lung Tissue of Asthmatic Rats

作　　者：江庆澜[1] 雷秀霞[1] 梁成结[2] 连家燕[2] 巫小莉[1] 刘桂长[1] 梁颜笑[1] 

机构地区：[1]广州市第一人民医院,510180 [2]广州医学院,510182

出　　处：《医学研究杂志》2010年第7期62-65,共4页Journal of Medical Research

基　　金：广州市医药卫生科技项目(2008-YB-027);广州市中医药;中西医结合科技项目(2009-A-05)

摘　　要：目的分析虎杖苷吸入剂对支气管哮喘大鼠模型TNF-α和Eotaxin基因表达调控的作用机制和干预效果。方法以10%的卵蛋白(OVA)和3%的氢氧化铝混合液腹腔注射实验大鼠2次致敏,然后以2%的OVA诱喘,并进行虎杖苷和氨茶碱干预及生理盐水对照实验两周。通过RT-QPCR方法检测各组实验大鼠肺组织TNF-α mRNA和Eotaxin mRNA的相对水平,以t检验统计分析各项实验数据。结果虎杖苷干预组大鼠的哮喘症状得到明显缓解,其肺组织TNF-α和Eotaxin mRNA的相对水平(0.0005±0.00039,0.0006±0.00035)比哮喘对照组(0.0165±0.01268,0.0280±0.02488)和正常对照组(0.0017±0.00101,0.0055±0.00209)均有所下降,但差异不显著(P>0.05,P>0.05;P>0.05,P>0.05)。结论虎杖苷吸入剂可降低哮喘大鼠肺组织的TNF-α和Eotaxin基因表达水平,有助于改善哮喘症状。ObjectiveTo analyze the effects and regulating mechanisms of TNF-α and Eotaxin gene expression of bronchial asthma rats intervened with polydatin inhalation. MethodsThe rats were injected with 1ml of the mix solution of 10% OVA and 3% aluminum hydroxide twice. And then, they were induced bronchial asthma with 2% OVA, and intervened with the polydatin solution and aminophyllin injection or controlled with saline for 2 weeks. The relative level of TNF-α and Eotaxin mRNA was detected by the methods of reverse transcription （RT） and real-time quantitative polymerase chain reaction （Real-time qPCR）. The data differences between the groups were analyzed by t-test. ResultsThe asthmatic symptom of rats in polydatin intervenient group was obviously improved. Their relative level of TNF-α and eotaxin mRNA in lung tissue （0.0005±0.00039, 0.0006±0.00035） was lower than those of asthmatic group （0.0165±0.01268, 0.0280±0.02488） and control group （0.0017±0.00101, 0.0055±0.00209）, but these differences between the groups were not significant （P〉0.05, P〉0.05; P〉0.05, P〉0.05）. ConclusionThe polydatin inhalation can decrease the TNF-α and eotaxin gene expression in the lung tissue of asthmatic rats to improve their asthmtic symptom.

关 键 词：支气管哮喘 虎杖苷 肿瘤坏死因子α 嗜酸性粒细胞趋化因 子基因表达 

分 类 号：R562.25[医药卫生—呼吸系统]