紫外可见分光光度法测定蒙药述达格-4中总黄酮的含量  

DETERMINATION OF TOTAL FLAVONE CONTENT IN Shudage-4 BY UV-VISIBLE SPECTROPHOTOMETRY

作　　者：贾鑫[1] 董玉[1] 武娜[1] 

机构地区：[1]内蒙古医学院药学院,内蒙古呼和浩特010059

出　　处：《内蒙古医学院学报》2010年第3期303-305,共3页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自治区科技厅项目(2008);内蒙古自治区高等学校科学研究项目(NJ10137)

摘　　要：目的：建立述达格-4中总黄酮含量的方法。方法：采用紫外可见分光光度法测定,检测波长为525nm。结果：高良姜素在浓度192.8~771.2μg/mL范围内呈良好线性关系。回归方程Y=0.7454X＋0.0145（r=0.9998）。线性关系良好,加样回收率为98.24%,RSD为2.17%。结论：该方法简便、灵敏、准确,可用于述达格-4中总黄酮的含量测定。Objective：To establish the content determination method for total flavone in Shudage-4.Methods：using Ultraviolet and visible spectrophotometric determination to detect at wavelength of 525 nm.Results：Galangin had a liner relationship with the Absorbance in the range of 192.8~771.2μg/mL.The regression equation was Y=0.7454X ＋0.0145（r=0.9998）.The average recovery for Galangin was 98.24%,RSD was 2.17%.Conclusion：The method is simple,sensitive,accurate and can be used for total flavones determination in Shudage-4.

关 键 词：述达格-4 总黄酮 紫外可见分光光度法 

分 类 号：R962[医药卫生—药理学]