高效液相色谱法测定蒙药升阳十一味丸中胡椒碱的含量  

DETERMINATION OF PIPERINE IN SHENGYANG SHIYIWEI PILL BY RP-HPLC

作　　者：温爱平[1] 王玉华[1] 杨来秀[1] 

机构地区：[1]内蒙古医学院药学院,内蒙古呼和浩特010059

出　　处：《内蒙古医学院学报》2010年第3期306-308,共3页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自治区教育厅资助项目(01082)

摘　　要：目的：建立蒙药升阳十一味丸中胡椒碱的含量测定方法。方法：采用高效液相色谱法,色谱柱：ODS柱,流动相：甲醇-水（77∶23）,检测波长：343nm。结果：胡椒碱在0.136~0.68μg范围内呈良好的线性关系,加样平均回收率为99.44%,RSD为1.67%。结论：本法可作为该药品的质量控制方法。Objective：Establishing a method of detecting piperine in Shengyang shitiwei pill.Methods：RP-HPLC method was used.The chromatographic conditions were listed below：using a C18 column,a mobile phase of methanol-water（77：23）,the detecting wavelength of 343 nm.Results： Piperine showed good linear relationship in the range of 0.136～0.68μg.The average recovery was 99.44%,RSD was 1.67%.Conclusion： The method can be used quality control of Shengyang shiyiwei pill.

关 键 词：高效液相色谱法 升阳十一味丸 胡椒碱 

分 类 号：R284.2[医药卫生—中药学]