黔北麻羊生长分化因子9和骨形态发生蛋白15基因遗传变异分析  

GDF9 and BMP15 Genes Analysis of Genetic Variation on Qianbei-pockmarked Goats

作　　者：任珍珍[1] 蔡惠芬[1] 罗卫星[1] 刘若余[1] 

机构地区：[1]贵州大学动物科学学院,贵州省动物遗传育种与繁殖重点实验室,贵阳550025

出　　处：《中国畜牧兽医》2010年第7期99-102,共4页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省农业科技攻关项目(黔科合NY字(2008)3044)

摘　　要：试验将生长分化因子9(GDF9)基因和骨形态发生蛋白15(BMP15)基因作为候选基因,采用直接测序法检测GDF9和BMP15基因在黔北麻羊中的单核苷酸多态性。结果表明,在GDF9基因外显子2的562 bp处发生了突变(A→C),导致谷氨酰胺突变为脯氨酸,检测到AA、Aa 2种基因型,基因型频率分别为0.7273、0.2727;A、a等位基因频率分别为0.8637、0.1363。BMP15基因外显子2的480 bp处发生了突变(C→G),导致谷氨酰胺突变为谷氨酸,检测到BB、Bb 2种基因型,基因型频率分别为0.7000、0.3000;B、b等位基因频率分别为0.8500、0.1500。Growth differentiation factor 9（GDF9）gene and bone morphogenetic protein 15（BMP15） gene as candidate genes on the prolificacy.Single nucleotide polymorphism of GDF9 gene and BMP15 gene was detected in Qianbei-pockmarked goats by sequencing.The results showed that there was one single nucleotide mutation（A562→C562） at exon 2 of GDF9 gene in Qianbei-pockmarked goats,and this mutation resulted in an amino acid change： glutamine→proline.The results indicated that there were two genotypes（AA and Aa） detected in Qianbei-pockmarked goats,genotypic frequency was respectively 0.7273 and 0.2727;frequency of A and a allele was 0.8637 and 0.1363.The results showed that there was one single nucleotide mutation（C480→G480）at exon 2 of BMP15 gene in Qianbei-pockmarked goats,and this mutation resulted in an amino acid change： glutamine→glutamate.The results indicated that there were two genotypes（BB and Bb） detected in Qianbei-pockmarked goats,genotypic frequency was respectively 0.7000 and 0.3000;frequency of B and b allele were 0.8500 and 0.1500.

关 键 词：GDF9基因 BMP15基因 遗传变异 黔北麻羊 

分 类 号：Q78[生物学—分子生物学]