绒山羊X染色体7个微卫星标记的遗传连锁图谱的构建  

A Linkage Map of 7 Microsatellite Markers on Chromosome X in Cashmere Goats

作　　者：徐磊[1,2] 姚继广 杨子森 赖双英[1] 张文广[1,2] 李金泉[2] 吴萍[1,2] 王志新[1,2] 乔峰[4] 王敏[1,2] 呼都特[1] 孟克格日乐[1,2] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,呼和浩特010018 [2]内蒙古自治区动物遗传育种与繁殖重点实验室,呼和浩特010018 [3]山西省生态畜牧产业管理站,太原030002 [4]内蒙古白绒山羊种羊场,鄂尔多斯017000

出　　处：《中国畜牧兽医》2010年第7期109-112,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划(2007BAD56B3)

摘　　要：本研究利用内蒙古白绒山羊的7个父系半同胞家系共794个个体,用X染色体上的7个微卫星标记进行了系谱确认,构建了绒山羊X染色体遗传连锁图。结果表明,7个标记的等位基因数变化范围为9～14,杂合度在0.585～0.918之间,平均杂合度为0.726;多态信息含量在0.759～0.897之间,平均多态信息含量为0.834。构建的内蒙古白绒山羊X染色体遗传连锁图总长度139.4 cM,与英国罗斯林研究所公布的SM4.7绵羊连锁图标记顺序一致,可用于下一步的QTL定位研究。The 7 microsatellite markers on chromosomes X were used to construct a linkage map of Cashmere goats with a 794 members half-sib pedigrees after paternity testing.The results showed that the number of alleles for 7 markers was 9 to 14,the heterozygosity was 0.585 to 0.918,the average heterozygosity was 0.726,and polymorphic information content（PIC） was 0.759 to 0.897,the average polymorphic information content was 0.834.Length of the linkage map of chromosome X we built was 139.4 cM,and the sequence of loci was coincident with the SM4.7,and could be used in quantitative trait loci（QTL） searching in Cashmere goats.

关 键 词：绒山羊 微卫星标记 X染色体 连锁图 

分 类 号：Q755[生物学—分子生物学]