南京地区性病患者沙眼衣原体感染的基因分型  

Genotyping of Chlamydia trachomatis Isolated from Patients with Sexually Transmitted Diseases in Nanjing Area.

作　　者：李珊山[1,2] 叶顺章[1,2] 

机构地区：[1]白求恩医科大学第一临床学院皮肤科 [2]中国医学科学院皮肤病研究所

出　　处：《中国皮肤性病学杂志》1999年第2期95-97,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的了解南京地区性病患者泌尿生殖道沙眼衣原体感染分离株的基因型（血清型）分布情况。方法应用聚合酶链反应和限制性片段长度多态性分析，对来自南京地区性病门诊病人和性乱妇女的９４份沙眼衣原体阳性标本进行了基因分型研究。结果Ｅ型为南京地区性病高危人群最流行的沙眼衣原体基因型，占３６．２％，其次为Ｆ、Ｇ和Ｄ型，分别占１９．２％、１７．０％和１２．８％，而Ｋ、Ｊ、Ｉ、Ｈ型比较少见。发现了一个Ｂ基因型，一个Ｆ／Ｇ混合型和４个非典型基因型。结论我国南京泌尿生殖道沙眼衣原体感染流行株的基因型分布情况与国外基本相同，且存在某些基因型的变异。Objective To understand the distribution of gene types (serovars) of isolates from patients with sexually transmitted Chlamydial trachomatis urogenital infections in Nanjing area.Methods A polymerase chain reaction and a restriction fragment length polymorphism were used to genotype 94 Chlamydial culture-positive clinical samples from patients attending sexually transmitted disease clinics and sexually promicuous women in Nanjing ares.Results The most prevalent genotype in this group of people was E(36.2%),following by F(19.2%),G(17.0%)and D(12.8%).Only a few strains from K,J,I and H were identified.In addition,one B,one F/G and four atypical genotypes were determined.Conclusion This study indicates that the distribution of genotypes of urogenital Chlamydia trachomatis isolates in Nanjing,China,is similar to that of other countrys in the world.And there exists genetic variation in some Chlamydial isolates in this country.

关 键 词：沙眼衣原体 基因分型 性病 

分 类 号：R759[医药卫生—皮肤病学与性病学]