骨细胞与成骨细胞诱导破骨细胞分化的对比研究  

Comparison between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in vitro

作　　者：崔亮[1] 李小彤[1] 杨雁琪[1] 傅民魁[1] 张丁[2] 

机构地区：[1]北京大学口腔医学院·口腔医院正畸科,100081 [2]中国医学科学院中国协和医科大学北京协和医院口腔科

出　　处：《中华口腔医学杂志》2010年第7期411-415,共5页Chinese Journal of Stomatology

基　　金：教育部高等学校博士学科点专项科研基金（20050001114）

摘　　要：目的 比较骨细胞和成骨细胞对破骨细胞分化形成的支持作用,初步探讨骨细胞在骨改建过程中的作用.方法 以小鼠骨髓基质细胞单独培养为空白对照组,以小鼠颅顶骨来源的成骨细胞与小鼠骨髓基质细胞共培养为成骨细胞组,以MLO-Y4骨细胞与小鼠骨髓基质细胞共培养为骨细胞组.使用骨吸收促进因子维生素D3处理3组细胞,抗酒石酸磷酸酶（tartrat resistant acid phosphatase,TRAP）染色后比较维生素D3处理前后3组破骨细胞数量的差异.结果 维生素D3处理前空白对照组破骨细胞计数为（6.0±1.O）个/孔板;成骨细胞组破骨细胞计数为（12.7±5.5）个/孔板,两组差异有统计学意义（P〈0.05）;骨细胞组破骨细胞计数为（1963.3±93.1）个/孔板,与其他两组间差异均有统计学意义（P〈0.001）.维生素D3对3组破骨细胞的分化形成均有促进作用.结论 在没有骨吸收促进因子存在的情况下,成骨细胞无法单独诱导破骨细胞分化,而MLO-Y4骨细胞可单独促进破骨细胞分化.骨吸收促进因子维生素D3可加强成骨细胞和骨细胞诱导破骨细胞分化的能力.Objective To compare between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in co-culture system.Methods MLO-Y4 cells and murine osteoblast cells were co-cultured with bone marrow cells with or without vitamin D3 presence.Bone marrow cells were as control group.Tartrat resistant acid phosphatase（TRAP） ＋ giant cells with three or more nuclei were counted and compared under a microscope at day 9.Results In the absence of vitamin D3,（1963.3±93.1）/plate osteoclasts were observed when MLO-Y4 cells co-cultured with bone marrow cells in 24-well plate.While only （12.7±5.5）/plate osteoclasts were found in the osteeblast group,and （6.0±1.O）/plate in control group.The statistical difference occurs for any two groups（P 〈0.05）.Vitamin D3 could significantly increase osteoclast formation in the three groups.Conclusions Osteocytes could induce osteoclastogenesis without the presence of vitamin D3 and vitamin D3 could enhance the induction effects of MLO-Y4 and ostenblast cells.

关 键 词：骨细胞 成骨细胞 破骨细胞 维生素D3 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]