新型磷灰石、硅灰石生物活性玻璃陶瓷-半水硫酸钙复合材料的制备及研究  

Study and preparation of a novel apatite-wollastonite bioactive glass-ceramic-calcium sulphate

作　　者：陈欣欣[1] 廖运茂[2] 朱智敏[1] 

机构地区：[1]四川大学华西口腔医学院修复科,成都610041 [2]四川大学口腔疾病研究国家重点实验室

出　　处：《中华口腔医学杂志》2010年第7期439-443,共5页Chinese Journal of Stomatology

摘　　要：目的 研制一种新型磷灰石-硅灰石生物活性玻璃陶瓷-半水硫酸钙（apatite-wollastonite bioactive glass-ceramic-calcium sulphate hemihydrate,AW-BGC-CSH）复合材料,探讨其作为骨组织工程支架材料的可行性.方法 按AW-BGC与医用半水硫酸钙（calcium sulphate hemihydrate,CSH）的不同质量百分数（CSH分别占50%、40%、30%、20%）制备复合材料,研究其形态结构及力学性能;并将复合材料与成骨细胞体外复合培养,通过形态学观察以及检测细胞增殖、碱性磷酸酶（alkaline phosphatase,ALP）活力及微量蛋白含量等指标,评价AW-BGC-CSH复合材料与成骨细胞间的相互作用.以单纯培养基作为空白对照组.结果 复合材料为三维立体多孔结构,可在3-5 min内保持其可塑性,最高固化温度为36.4℃,抗压强度最高可达9.3 Mpa.成骨细胞在复合材料上生长良好,实验组复合材料的毒性级为0级.复合培养1、3、 5、 7 d后微量蛋白含量分别为（251±12）、（296±31）、（580±13）和（571±15）mg/L,ALP活性分别为（4.50±0.68）、（6.90±0.27）、（12.05±0.28）和（11.86±0.63）U/mg;与空白对照组相比,差异均有统计学意义（P〈0.05）.结论 新型AW-BGC-CSH复合材料具有三维立体结构、良好的可塑性和抗压强度,以及良好的生物相容性.Objective To prepare a novel apatite-wollastonite bioactive glass-ceramic-calcium sulphate hemihydrate（AW-BGC-CSH） composite,to study its biocompatibility,and to provide experimental support for its further clinical application.Methods Samples of AW-BGC-CSH composite were prepared with different AW-BGC granules-CSH ratios （50%,40%,30%,20%）.Surface morphology,microstructure and mechanical features of the composite were measured.Osteoblasts were cultivated in vitro on the composite.Cell morphology,proliferation,and the alkaline phosphatase（ALP） activity of osteoblasts were examined to determine the biocompatibility of the composite.Results The composite showed a threedimensional pored structure with communicated micropores under scanning electron microscopy（SEM）.The plasticity of the composite could be maintained within 3-5 min.Its top solidification temperature was 36.4 ℃and the maximum compressive strength was 9.3 Mpa.The osteoblasts adhered to the composite and grew well.At 1,3,5,7 d after cultivated,the microprotein contents of the composite were （251±12）,（296±31）,（580±13） and （571±15）mg/L,and the ALP activity of the composite were （4.50±0.68）,（6.90±0.27）,（12.05±0.28） and （11.86±0.63） U/mg.The results of the ALP activity and microprotein contents in the experiment group were significantly higher than those in the control group（P 〈0.05）.Conclusions The prepared AW-BGC-CSH composite has a three-dimensional pored structure,favourable plasticity,mechanical property and good biocompatibility.

关 键 词：硫酸钙 骨代用品 成骨细胞 磷灰石-硅灰石生物活性玻璃陶瓷 

分 类 号：R318.08[医药卫生—生物医学工程]