丝素-壳聚糖共混膜与颌下腺细胞体外复合培养的实验研究  

Investigation of silk fibroin-chitosan blend and submandibular glands cell co-culture in vitro

作　　者：王哲[1] 谭学新[1] 黄巍巍[1] 马丽[1] 李欣宇[1] 

机构地区：[1]中国医科大学口腔医学院颌面外科教研室,沈阳110001

出　　处：《口腔医学》2010年第7期406-409,共4页Stomatology

基　　金：辽宁省教育厅高等学校科研项目(2008843)

摘　　要：目的探讨丝素-壳聚糖共混膜(silkfibroin-chitosan blend,SFCS)与颌下腺细胞体外复合培养的可行性。方法体外原代培养SD大鼠颌下腺细胞(Rat submandibular gland cells RSMG-cell)并传代,将浓度为2.0×104个/ml的第2代颌下腺细胞接种于5mm×5mm×2mm丝素-壳聚糖共混膜上体外复合培养。观察颌下腺细胞附着率和增殖情况,扫描电镜观察细胞超微结构,分别取复合培养1～10d的培养上清液,测定淀粉酶含量。结果颌下腺细胞24h附着率大于90%,生长、增殖情况良好。扫描电镜下可见复合培养7d的颌下腺细胞在丝素-壳聚糖共混膜上伸展充分,表面有许多分泌颗粒。随着复合培养时间的延长,细胞上清液中淀粉酶含量有不同程度的增加。结论颌下腺细胞与丝素-壳聚糖共混膜复合培养,颌下腺细胞附着、增殖能力及分泌功能良好,丝素-壳聚糖共混膜可以作为颌下腺组织工程的支架材料进行进一步的研究。Objective To study the possibility of silk fibroin-chitosan blend （ SFCS） and rat submandibular glands （ RSMG-cell） outside co-culture.Methods We descended outside original generation development of the SD rat RSMG-cell and spread the generation development with 2.0 × 10^4/ml of the 2nd generation the RSMG-cell to 5 mm × 5 mm × 2 mm SFCS.We observed the RSMG-cell adherence rate and proliferation rate.With electronic scanning microscope we observed the cell super micro-structure and took co-cultured development of 1-10 day top pure liquid,respectively,measured the starch Mao content.Results The RSMG-cell 24h was more than 90% ,grew and proliferated well.With electronic scanning microscope we found that co-cultured 7-days RSMG-cell at the SF-CS top stretched well,and the surface had many secrete grains.With the extension of co-culture time,the starch Mao content increased to different degrees.Conclusions The RSMG cell and SFCS co-culture,the cell proliferation ability and secreting function were good, and SFCS could be the supporting material for the RSMG-cell tissue engineering to carry on further research.

关 键 词：颌下腺细胞 丝素-壳聚糖共混膜 复合培养 

分 类 号：R329.29[医药卫生—人体解剖和组织胚胎学]