Using DNA-based techniques to identify hybrids among linear-leaved Potamogeton plants collected in China  

作　　者：Zhi-Yuan DU Chun-Feng YANG Jin-Ming CHEN You-Hao GUO Akeem Babalola KADIRI 

机构地区：[1]Key Laboratory of Aquatic Botany and Watershed Ecology, Chinese Academy of Sciences, Wuhan 430074, China [2]Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China [3]Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China [4]Department of Botany and Microbiology, University of Lagos, Lagos 100213, Nigeria

出　　处：《Journal of Systematics and Evolution》2010年第4期265-270,共6页植物分类学报（英文版）

基　　金：supported financially by the National Natural Science Foundation of China(grants 30430050 and 30800061)

摘　　要：It is well known that interspecific hybrids occur in the genus Potamogeton. The linear-leaved Potamogeton species commonly have highly variable morphological characteristics. Their hybrids often show similar vegetative characters to their parental species and their identification based solely on morphology is not always conclusive. In order to clarify whether there are any hybrids from the linear-leaved Potamogeton plants collected in China, we used internal transcribed spacers （ITS） of nuclear ribosomal DNA and chloroplast rbcL gene sequences to identify the hybrids. Using ITS sequence additivity, we identified four hybrids, namely P. orientalis （P. pusillus × P. oxyphyllus）, P pusillus × P. berchtoldii, P foliosus × P octandrus, and P. cristatus × P octandrus. The latter three hybrids should be considered as new hybrids in Potamogeton. The maternal parents of the four hybrids were confirmed using chloroplast rbcL gene sequences.It is well known that interspecific hybrids occur in the genus Potamogeton. The linear-leaved Potamogeton species commonly have highly variable morphological characteristics. Their hybrids often show similar vegetative characters to their parental species and their identification based solely on morphology is not always conclusive. In order to clarify whether there are any hybrids from the linear-leaved Potamogeton plants collected in China, we used internal transcribed spacers （ITS） of nuclear ribosomal DNA and chloroplast rbcL gene sequences to identify the hybrids. Using ITS sequence additivity, we identified four hybrids, namely P. orientalis （P. pusillus × P. oxyphyllus）, P pusillus × P. berchtoldii, P foliosus × P octandrus, and P. cristatus × P octandrus. The latter three hybrids should be considered as new hybrids in Potamogeton. The maternal parents of the four hybrids were confirmed using chloroplast rbcL gene sequences.

关 键 词：HYBRIDIZATION internal transcribed spacer POTAMOGETON RBCL 

分 类 号：Q949.758.8[生物学—植物学] S562.035.1[农业科学—作物学]