高效液相色谱法测定松针中莽草酸的含量  

Determination of the content of shikimic acid in pine needles by high performance liquid chromatography

作　　者：谢济运[1,2] 陈小鹏[1] 粟本超[1] 陈芳[1] 李志荣[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004 [2]柳州师范高等专科学校,广西柳州545004

出　　处：《化学研究与应用》2010年第7期943-947,共5页Chemical Research and Application

基　　金：国家自然科学基金资助项目(30560119);广西自然科学基金资助项目(0991030);广西研究生教育创新计划项目(2008105930817M46);广西教育厅科学研究项目([2006]26);广西教育厅科学研究项目(200708LX180);广西大学科研基金项目(X081020);广西青年基金项目(0832008)

摘　　要：松针为我国传统中药，《本草纲目》曰：松针，味苦温、无毒，久服令人不老，轻身益气，主治风湿疮，生毛发，安五脏，守中，不饥，延年。历代均入药用，民间用于治疗神经衰弱、维生素丙缺乏症、营养性水肿、风牙肿痛、阴囊湿痒、腰痛、跌打损伤和预防钩虫病。现代临床医学上常用以治疗慢性气管炎、预防流脑和感冒。The content of shikimic acid in Pinus elliottii needles were determined by high performance liquid chromatography（HPLC）,samples extracted with petroleum ether defatted after distillation of water,then extraction and purification by using n-butanol and 5% NaOH,respectively,and finally carry out separation and detection by HPLC.The mobile phase was Hexane 2%H3PO4（90∶10）;the flowing velocity was l ml/min;Alltima NH2（5 μm,4.6 mm×150 mm） column separation,test wavelength was 213 nm.Shikimic acid in 0.33 ~ 3.3 μg/μL range of linear relationship,correlation coefficient of 0.9992.Shikimic acid to be the average recovery was 98.5%.RSD=0.85%（n=5）.Pinus elliottii needles of dry extraction of shikimic acid in the average rate of 1.32%.

关 键 词：高效液相色谱法 松针 莽草酸 

分 类 号：O657.7[理学—分析化学]