糖皮质激素对兔淋巴细胞β受体的影响  

The Action of Glucocorticoids on Beta-adrenoceptors of Rabbit Lymphocytes

作　　者：敖屹 黎明涛[1] 夏宗勤[2] 易宁育[2] 

机构地区：[1]上海第二医科大学细胞调控研究室 [2]上海第二医科大学

出　　处：《上海第二医科大学学报》1990年第4期289-291,共3页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：作者用自行标记的^(125Ⅰ)-(±)心得静作特异配基测定家兔外周血淋巴细胞β-肾上腺素能受体(β-AR)在糖皮质激素作用前后的变化情况。实验测得正常情况下家兔血淋巴细胞β-AR最大结合容量Bmax=1.36±0.36 fmol/10~6cells,KD=0.16±0.04nM,n=5。注射氢化泼尼松后Bmax=2.11±0.36fmol/10~6cells,KD=0.17±0.02nM,T检验表明两组Bmax值有显著差异,而KD无显著差异;停药三周复测,Bmax=1.22±0.22fmol/10~6cells,KD=0.18±0.07nM,表明已恢复到用药前的水平。The effect of prednisolone on beta-adrenoceptors of rabbit circulating lymphocytse was studied with ^(125)I-pindolol binding assay.24 hours after a single muscular dose(2 mg/kg)of prednisolone, the density of binding sites RT was significantly higher than before injection (2.11±0.36, 1.36±0.36 fmol/10~6 cells respectively), It returned to normal range three weeks later (1.22±0.22fmol/10~6 cells). The equilibrium dissociation constant KD showed no marked change throughout the experiment. Since it is well known that glucocorticoids can increase the density of visceral beta-adrenoceptors, the result of this study provides an evidence that the changes of beta-adrenoceptors on circulating lymphocytes may reflect those in visceral tissues.

关 键 词：糖皮质激素 淋巴细胞 Β受体 兔 

分 类 号：R965.1[医药卫生—药理学]