石菖蒲提取成分对大鼠脑皮质NMDA、M受体与其配体结合的影响  

Effects of Rhizoma Acori Tatarinowii Extract on the Binding of Cerebral Cortical NMDA or M Receptors with Their Ligands

作　　者：匡忠生[1] 江湧[1] 李志强[2] 王淑英[1] 谢宇晖[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405 [2]广州中医药大学,广州510405

出　　处：《中药新药与临床药理》2010年第4期354-357,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省高等学校中药有效性与安全性重点实验室开放基金项目(kf08006)

摘　　要：目的观察石菖蒲水提取成分对大鼠脑皮质NMDA、M受体与其配体结合的影响。方法提取脑皮质受体后,加入相应的石菖蒲提取成分,运用放射配基结合分析法检测NMDA、M受体与其相应配体的的结合。结果石菖蒲挥发油、去油水煎液、β-细辛醚均能拮抗NMDA受体与3H-MK 801的结合,但对M受体与3H-QNB的结合无影响。结论石菖蒲水提成分能拮抗大鼠脑皮质NMDA受体与其配体的结合,该作用可能是其醒脑开窍、治疗脑损伤的重要药理机制之一。Objectives To observe effects of water-extract of Rhizoma Acori Tatarinowii on the bindings of rats cere-bral cortical NMDA or M receptors with their ligands.Methods We extracted rats' cerebral cortical receptors firstly,and then added extracts of Rhizoma Acori Tatarinowii into receptors.Finally we detected changes of cerebral NMDA or M receptors binding with their ligands by radioligand binding assay.Results β-asarone,volatile oil and deoiling de-coction of Rhizoma Acori Tatarinowii all inhibited the binding of NMDA receptor with 3H-MK-801,but had no effect on the binding of M receptor with 3H-QN-B.Conclusion Extracts of Rhizoma Acori Tatarinowii can inhibit the binding of NMDA receptor with its ligand,which may be one of its pharmacological mechanisms in curing cerebral injury.

关 键 词：石菖蒲 脑皮质 NMDA受体 M受体 放射配基结合分析法 大鼠 

分 类 号：R285.5[医药卫生—中药学]