不同品系大鼠对天花粉蛋白诱导被动皮肤过敏差异性的比较研究  

Study on Variability of Passive Cutaneous Anaphylaxis Induced by Trichosanthin Among Three Rat Strains

作　　者：李秀芳[1] 金若敏[1] 符胜光[1] 姚广涛[1] 谭梦晖[1] 

机构地区：[1]上海中医药大学药物安全性评价研究中心,上海201203

出　　处：《中药新药与临床药理》2010年第4期382-385,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家十一五科技支撑计划项目(2006BAI06A03-02);国家"重大新药创制"科技重大专项项目(2009ZX0902-002)

摘　　要：目的比较SD、Wistar和F344三种品系大鼠对天花粉蛋白诱导的被动皮肤过敏(Passive cutaneous ana-phylaxis,PCA)反应的差异性。方法选用SD、Wistar和F344三种品系大鼠,用天花粉蛋白为致敏原,以PCA反应时出现蓝斑的直径及蓝斑OD值变化率作为判别标准,进行大鼠同种或小鼠异种PCA试验。结果大鼠同种PCA试验显示,每只大鼠注射天花粉蛋白0.9 mg后,三种品系大鼠PCA反应均为阳性,蓝斑直径大小及蓝斑OD值变化率从大到小依次为:SD、F344大鼠>Wistar大鼠;小鼠异种PCA试验显示,耳廓皮内分别注射三种品系大鼠天花粉蛋白抗血清后,小鼠异种PCA反应均为阳性,耳廓蓝染OD值变化率从大到小依次为:SD大鼠抗血清组>F344大鼠抗血清组>Wistar大鼠抗血清组。结论三种品系大鼠对天花粉蛋白的敏感性存在差异,其中以SD大鼠最为敏感。Objective To compare the variability of passive cutaneous anaphylaxis （PCA） caused by trichosanthin （TCS） among Sprague Dawley（SD）, Wistar and Fischer344（F344） rats. Methods Homologous PCA was performed on SD, Wistar and F344 rats, and xenogenic PCA was performed on KM mice. TCS was taken as the allergen. The diameter of dermal Evan＇s blue spot and the optical density were measured to evaluate the severity of PCA. Results The results of homologous PCA showed that PCS was positive in all of the three rat strains after injection of 0.9mg TCS for each rat. According to the the diameter of Evan＇s blue spots and the optical density, PCA was stronger in SD and F344 rat than that in Wistar rat. Xenogeneie PCA was positive in KM mice after intradermally auricular injection of TCS antiserum of the three different rat strains. According to the optical density of Evan＇s blue spots, the intensity of antiserum was as follows： SD rat antiserum〉 F344 rat antiserum 〉 Wistar rat antiserum. Conclusion There are variabilities in sensitivity of TCS-induced PCS among the three rat strains, and SD rat is the most sensitive one.

关 键 词：天花粉蛋白 被动皮肤过敏 大鼠 品系 

分 类 号：R285.5[医药卫生—中药学]