独一味药材中环烯醚萜苷类成分指纹图谱研究  

HPLC Fingerprint of Iridoid Glycosides in Lamiophlomis rotat Kudo.

作　　者：尉丽力[1,2] 李茂星[1] 樊鹏程[1] 张汝学[1] 张泉龙[1] 贾正平[1,2,3] 

机构地区：[1]兰州军区兰州总医院药材科,兰州730050 [2]兰州大学药学院,兰州730000 [3]兰州大学生命科学学院,兰州730000

出　　处：《中药新药与临床药理》2010年第4期403-406,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：全军中医药研发推广项目(2006032001);甘肃省科技攻关项目(2GS054-A43-014-14)

摘　　要：目的建立独一味药材中环烯醚萜苷类成分HPLC指纹图谱分析方法,比较不同产地、不同年份的独一味药材中环烯醚萜苷类成分指纹图谱特征,为科学评价与有效控制独一味药材的质量提供参考。方法色谱柱为Symmetry C18(4.6 mm×250 mm,5μm,Waters),流动相为30%甲醇,pH=4.50,流速为1.0 mL.min-1,柱温为室温,检测波长为238 nm。结果建立了具有14个共有峰的独一味药材的环烯醚萜苷类成分指纹图谱,各色谱峰分离较好,达到指纹图谱要求。结论色谱指纹图谱分析方法简便,可为独一味药材质量评价提供依据。Objective To establish a HPLC fingerprint analysis method for the identification of iridoid glycosides in Lamiophlomis rotata Kudo. （LRK）, and to compare fingerprints of LRK from different habitats and different years, so as to establish a sensitive and specific method for quality controle of LRK. Methods HPLC analysis was performed on Symmetry C18 column （4.6 ×250mm,5μm, Waters）, and mixture of methanol-water （30 ： 70, pH 4.5）was used as the mobile phase at a flow rate of 1.0 mL·min-j. The column temperature was at room temperature and the detection wavelength was 238 nm. Results The mutual mode of HPLC-FPC fingerprint with 14 common peaks was set up. Peaks in the spectra were all separated perfectly, which met the regulation of HPLC-FPC. Conclusion The established HPLC fingerprint analysis method is simple, and can be used for quality control of LRK.

关 键 词：独一味 环烯醚萜苷 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]