云南省恶性疟原虫对氯喹、氨酚喹、哌喹、甲氟喹、奎宁敏感性的体外测定  

ASSAY OF SENSITIVITY OF PLASMODIUM FALCIPARUM TO CHLOROQUINE,AMODIAQUINE,PIPERAQUINE, MEFLOQUINE AND QUININE IN YUNNAN PROVINCE *

作　　者：杨恒林[1,2,3] 刘德全[1,2,3] 黄开国[1,2,3] 杨亚明 杨品芳[1,2,3] 廖明铮 张春勇[1,2,3] 

机构地区：[1]云南省疟疾防治研究所 [2]中国预防医学科学院寄生虫病研究所 [3]云南省瑞丽市卫生防疫站

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第1期43-45,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：联合国计划开发署/世界银行/世界卫生组织热带病研究培训特别规划处(WHO/TDR)及卫生部青年科学基金

摘　　要：目的：了解云南省恶性疟原虫对氯喹、氨酚喹、哌喹、甲氟喹及奎宁的敏感性。方法：采用Ｒｉｅｃｋｍａｎｎ体外微量法测定采自云南省瑞丽１１个县、市的恶性疟原虫对以上药物的敏感性。结果：云南省南部、东南部及西部恶性疟原虫对氯喹抗性率分别为９６．７％、７８．９％及９５．７％，ＩＤ５０依次为１２５ｎｍｏｌ／Ｌ、１３６ｎｍｏｌ／Ｌ、及１７６ｎｍｏｌ／Ｌ；对氯酚喹的抗性率分别为１００％、８５．３％及８８．９％，ＩＤ５０依次为５２ｎｍｏｌ／Ｌ、５４ｎｍｏｌ／Ｌ及７２ｎｍｏｌ／Ｌ；对奎宁均为敏感，ＩＤ５０依次为４８０ｎｍｏｌ／Ｌ、３５２ｎｍｏｌ／Ｌ及６０８ｎｍｏｌ／Ｌ。南部及东南部原虫对哌喹的抗性率分别为６８及８８ｎｍｏｌ／Ｌ；结论：云南省恶性疟原虫对４－氨基喹啉类药物普遍产生抗性，其抗性程度来自滇西及其相连的缅甸感染的疟原虫明显高于滇东南；对奎宁及甲氟喹敏感。氯喹。AIM:To determine the sensitivity of P.falciparum to chloroquine,amodiaquine,piperaquine,mefloquine and quinine in Yunnan Province of China in 1992～1995.METHODS:Rieckmanns in vitro microtechnigue was used. The sensitivity of P.falciparum was tested to the above mentioned antimalarials.RESULTS: The resistance rates of isolates of P.falciparum from the south, southeast and the west part of Yunnan to chloroquine and amodiaquine were 96 7%(29/30),78 9%(30/38),95 7%(22/23) and 100%(30/30),85 3%(29/34),8/9, respectively,with their corresponding ID 50 of 125 nmol/L,136 nmol/L and 176 nmol/L, and 52 nmol/L,64 nmol/L and 72 nmol/L,respectively.All the isolates were sensitive to quinine and their ID 50 were 480 nmol/L ,352 nmol/L,608 nmol/L ,respectively. The resistance rates of P.falciparum from the south part and southeast part of Yunnan to piperaquine were 96 4%(27/29),72 9 (27/37),respectively,their ID 50 were 320 nmol/L and 228nmol/L;all the cases were sensitive to mefloqine,their ID 50 were 68 nmol/L and 88 nmol/L. CONCLUSION: P.falciparum generally produces resistance to chloroquine, amodiaquine and piperaquine in Yunnan Province; the degree of resistance to chloroquine of P.falciparum from the west part of Yunnan were higher than the P.falciparum from the southeast part of Yunnan;all the isolates were sensitive to mefloquine and quinine in this region.

关 键 词：恶性疟原虫 体外测定 疟疾 治疗 氯喹 氨酚喹 

分 类 号：R531.305[医药卫生—内科学]