RP-HPLC法测定人血浆中氟吡汀的浓度  

Determination of the concentration of flupirtine in human plasma by RP-HPLC

作　　者：刘龙兴[1] 夏东亚[2] 郭涛[2] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳军区总医院药剂科,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2010年第7期559-562,共4页Journal of Shenyang Pharmaceutical University

基　　金：全军医学科学技术研究"十一五"计划科技攻关课题基金资助项目(编号:06G023)

摘　　要：目的建立以HPLC法测定人血浆中马来酸氟吡汀的浓度的方法。方法采用DiamonsilC18色谱柱,流动相：5 mmol.L-1磷酸氢二钠缓冲溶液-乙腈（体积比为55∶45）,流速：1.0 mL.min-1,荧光检测激发波长：323 nm,发射波长：370 nm。结果氟吡汀血药质量浓度在10.0-1 500.0μg.L-1内线性关系良好（r=0.999）,定量下限为10μg.L-1,方法回收率平均为105.36%,提取回收率平均为90.74%,日内、日间RSD均小于10%。结论本方法适用于人血浆中氟吡汀的药代动力学研究。Objective To establish an HPLC method for the determination of flupirtine in human plasma.Methods The separation was carried on a Diamonsil C18 column with the mobile phase consisted of 5 mmol·L-1 phosphate buffer-acetonitrile（55∶45）;the flow rate was 1.0 mL·min-1 and the excitation and emission wavelengths were set at 323 and 370 nm,respectively.Results The linear range of flupirtine was 10.0-1 500.0 μg·L-1（r=0.999）,with the lower quantity limit of 10.0 μg·L-1.The average method recovery was 105.36% and the average extraction recovery was 90.74%.The inter-day RSD and intra-day RSD were both less than 10%.Conclusions The method is simple,sensitive,rapid and accurate,and can be used for the pharmacokinetic study of flupirtine.

关 键 词：氟吡汀 高效液相色谱法 血药浓度 

分 类 号：R917[医药卫生—药物分析学]