HPLC法同时测定白鲜皮中γ-崖椒碱和白鲜碱的含量  

Determination of γ-fagarine and dictamnine in Densefruit Pittany Root-bark by HPLC

作　　者：李倩[1] 贾凌云[1] 孙启时[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2010年第7期570-573,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立同时测定白鲜皮药材中2种生物碱含量的HPLC方法,并测定不同产地白鲜皮药材中生物碱的含量,为白鲜皮药材质量控制提供科学依据。方法色谱柱：Diamonsil C18柱（200 mm×4.6 mm,5μm）,流动相：乙腈-水（体积比为40∶60）,流速：1.0 mL.min-1,检测波长：240nm。结果γ-崖椒碱和白鲜碱能够达到基线分离。γ-崖椒碱质量浓度在2.4-72.0 mg.L-1（r=0.9998）内,白鲜碱质量浓度在7.2-216.0 mg.L-1（r=0.9997）内与峰面积呈良好的线性关系;γ-崖椒碱和白鲜碱的回收率分别为100.2%、99.9%,RSD分别为1.7%、1.5%。结论所建立的方法可用于白鲜皮的质量控制。Objective To establish a method to determine simultaneously γ-fagarine and dictamnine by HPLC and provide basis for the quality control of Densefruit Pittany Root-bark.Methods The separation was performed with a Diamonsil C18 column（200 mm×4.6 mm,5 μm）with mobile phase consisted of CH3CN-H2O（V∶V=40∶60）and detection at 240 nm.The flow rate was 1.0 mL·min-1.Results γ-fagarine and dictamnine showed good separation with above method.The line at ranges was 2.4-72.0 mg·L-1（r=0.999 8）for γ-fagarine and 7.2-216.0 mg·L-1（r=0.999 7）for dictamnine.The recoveries for those two standard substances were 100.2% and 99.9% with RSD 1.7% and 1.5%.Conclusions The established method can be used to control the quality of Densefruit Pittany Root-bark.

关 键 词：高效液相色谱法 白鲜皮 γ-崖椒碱 白鲜碱 含量测定 

分 类 号：R917[医药卫生—药物分析学]