菜豆晕疫病菌的检测技术研究  

Development for the detection of Pseudomonas syringae pv. phaseolicola

作　　者：崔汝强[1,2] 胡学难[2] 冯黎霞[2] 赵立荣[2] 钟国强[2] 

机构地区：[1]华南农业大学资源环境学院,广东广州510642 [2]广东检验检疫技术中心

出　　处：《植物检疫》2010年第4期25-27,共3页Plant Quarantine

基　　金：国家质检总局项目(2009IK262);科技部支撑项目(2006BAD08A16)

摘　　要：通过选择性培养基的筛选,利用菜豆萎蔫毒素基因序列扩增引物和菜豆萎蔫毒素基因缺失菌株独有的ORF6序列的扩增引物,采用双重PCR技术实现了菜豆晕疫病菌的快速检测。Selective medium and double-PCR were used to detect Pseudomonas syringae pv. phaseolicola. The primers P3004L and P3004R were used to amplify the ORF6 sequence of the strains of P. syringae pv. phaseolicola that lacked the phaseolotoxin gene,whereas primers PSP1F and PSP1R were used to amplify the gene sequence of the biosynthesis of phaseolotoxin. This method would be useful for rapid detection.

关 键 词：菜豆晕疫病菌 选择性培养基 双重PCR 检测 

分 类 号：S41-30[农业科学—植物保护]