利用TaqMan探针检测水稻细菌性谷枯病菌  

Using TaqMan probe for the detection of Burkholderia glumae

作　　者：李巍[1] 莫瑾[2] 彭梓[2] 姜金林[2] 朱金国[2] 

机构地区：[1]湖南农业大学,湖南长沙410128 [2]湖南出入境检验检疫局

出　　处：《植物检疫》2010年第4期32-34,共3页Plant Quarantine

基　　金：湖南农业大学青年基金(09QN15);国家质检总局课题(2003IK067;2006IK214);质检公益性行业科研专项(200810632)

摘　　要：利用TaqMan探针建立了水稻细菌性谷枯病菌(Burkholderia glumae)实时荧光PCR检测方法。根据水稻细菌性谷枯病菌gyrB基因,设计并合成特异性引物和探针,对8株不同来源水稻细菌性谷枯病菌和其他同属或同寄主的参试菌株进行了检测。结果显示,该方法检测的特异性强,灵敏度可达菌悬液浓度102cfu/mL,该方法快速、简便、准确,适用于出入境检验检疫及种子健康检测领域。利用该方法对国内采集的83份水稻材料进行了检查,未发现阳性结果。A real time-PCR method was developed for the detection of Burkholderia glumae with specific primers and TaqMan probe which were designed based on the region of gyrB gene. In this assay,for the 8 strains of B. glumae from different origins and other related 18 bacterial strains,fluorescent signals were only obtained from all the B. glumae with their specific probes,while other strains showed negative results. The sensitivity of the probe was 102 cfu/mL. 83 rice samples from China were detected using this method; the result showed that all the samples were negative. This method is little time consumption and without contamination from PCR product. This method provides as a specific,sensitive and rapid detection of Burkholderia glumae for the seed.

关 键 词：实时荧光PCR 水稻细菌性谷枯病菌 gyrB基因 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]