用二阶导数光谱法测定栀子中总环烯醚萜苷的含量  

Determination of the content of total iridoid glycoside in Gardenia jasminoides Ellis by second order derivative spectrophotometry

作　　者：王雷琛[1,2] 王秀鑫[1,2] 汪硕闻[1,2] 周婷婷[1,2] 范国荣[1,2] 

机构地区：[1]第二军医大学药学院药物分析学教研室,上海200433 [2]上海市药物代谢重点实验室,上海200433

出　　处：《药学服务与研究》2010年第3期205-207,共3页Pharmaceutical Care and Research

基　　金：国家自然科学基金资助项目(No.30801541);第二军医大学大学生创新能力培养基金

摘　　要：目的:建立测定栀子中总环烯醚萜苷含量的二阶导数光谱法,并用该方法测定不同产地栀子中总环烯醚萜苷的含量。方法:采用二阶导数光谱法在263nm波长处测定栀子中总环烯醚萜苷的含量。结果:栀子苷在4.32～21.60μg/ml范围内呈良好线性关系;其回归方程为A=2.986×10-5c-1.833×10-5(r=0.9996);回收率为(98.86±2.39)%(n=5),精密度、重现性和稳定性良好。结论:本方法操作简单、准确,可用于测定栀子中总环烯醚萜苷的含量。Objective：To establish a second order derivative spectrophotometry method for determination of the content of total iridoid glycoside in Gardenia jasminoides Ellis from different regions.Methods：The second order derivative spectrophotometry was performed at the wavelength of 263 nm.Results：A good linear relationship was obtained in the range of 4.32-21.60 μg/ml of geniposide.The regression equation was A=2.986×10-5 c-1.833×10-5（r=0.999 6）.The average recovery rate was （98.86±2.39）% （n=5）.Conclusion：The method is simple and accurate,and can be used for determination of total iridoid glycoside in Gardenia jasminoides Ellis.

关 键 词：栀子 环烯醚萜苷 分光光度法 紫外线 栀子苷 含量测定 

分 类 号：R927.2[医药卫生—药学]