南宁市散发性急性胃肠炎病例中检出GⅠ.8型诺如病毒  

Norovrius GenotypeⅠ.8 Being Detected from Sporadic Cases of Acute Gastroenteritis in Nanning Municipal

作　　者：邓丽丽[1] 刘巍[1] 谭冬梅[1] 杨哲[1] 谢镇国[1] 唐振柱[1] 黄兆勇[1] 方志锋[1] 杨进业[1] 董柏青[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530028

出　　处：《中国疫苗和免疫》2010年第3期241-245,共5页Chinese Journal of Vaccines and Immunization

基　　金：广西壮族自治区科学技术厅项目(桂科攻9732027;桂科攻0592007-4;桂科条08-05-01D);广西壮族自治区卫生厅科学研究课题(合同号:Z2008214;Z2008215)

摘　　要：目的研究南宁市某医院散发性急性胃肠炎病例中,GⅠ.8型诺如病毒(Norovirus,NV)的基因特征。方法采用荧光定量逆转录-聚合酶链反应,对2007年1月～2008年12月南宁市某医院散发性急性胃肠炎病例标本检测基因Ⅰ型(GenogroupⅠ,GⅠ)NV核糖核酸,对阳性标本进行N/S区核苷酸序列测定,并构建系统进化树分析。结果在696份粪便标本中,检出3份GⅠ型NV核酸阳性。经核酸序列分析发现,其中一份[南宁(Nanning,NN)07230]与GⅠ.8型参考株(WUGⅠ/00/JP)的核苷酸序列同源性达94.5%;与2006～2007年日本、韩国公布的GⅠ.8型NV核苷酸序列同源性范围在96.7%～98.5%。NN07230在进化树中与GⅠ.8型病毒株属同一分支。结论在南宁市散发性急性胃肠炎病例中存在GⅠ.8型NV感染。Objective To study the genic characteristic of Norovrius genotypeⅠ.8 from sporadic gastroenteritis of a hospital in Nanning Municipal.MethodsSpecimens of diarrhea patients from a hospital in Nanning Municipal between January 2007 and December 2008 were collected and detected for Noroviral genogroupⅠribonucleic acid（RNA） by real-time reverse transcription-polymerase chain reaction（real-time RT-PCR）.All positive specimens were subjected for sequencing analysis and constructed phylogenetic tree.ResultsNorovirus genogroupⅠRNA were detected in 3 of 696 stool specimens.The homology between one of the four strains（NN07230） and reference strain of GⅠ.8（WUGⅠ/00/JP） was 94.5%;and the homology between the NN07230 strain and strains circulating during 2006-2007 in Japan and Korea ranged from 96.7% to 98.5%.And they formed one cluster in the phylogenetic tree.ConclusionThe research showed that genotypeⅠ.8 infection exists in sporadic gastroenteritis in Nanning City.

关 键 词：诺如病毒 散发性急性胃肠炎 GⅠ.8型 序列分析 

分 类 号：R373.2[医药卫生—病原生物学]