奶牛隐性乳房炎多重PCR检测方法研究  

Multiple PCR for Detection of Subclinical Mastitis in Dairy Cow

作　　者：肖颖[1] 谷维娜[1] 钱明明[2] 刘磊[2] 赵宝华[2] 

机构地区：[1]石家庄学院化工学院,河北石家庄050035 [2]河北师范大学生命科学学院,河北石家庄050016

出　　处：《安徽农业科学》2010年第17期9029-9031,9112,共4页Journal of Anhui Agricultural Sciences

基　　金：石家庄市科技攻关项目(20091126A)

摘　　要：[目的]建立一种快速、准确和特异性强的奶牛隐性乳房炎检测方法。[方法]采用多重PCR从基因水平上对70份临床疑似奶牛隐性乳房炎感染样本进行检测。[结果]多重PCR方法检测隐性乳房炎阳性样品数为58份,阳性检出率为82.5%;传统生化方法检测阳性样品数为53份,阳性检出率为75.5%,两者的阳性符合率为92%。[结论]成功地建立了一种快速、特异性强的奶牛隐性乳房炎多重PCR检测方法。[Objective]To establish a rapid,specific and accurate diagnostic method for subclinical mastitis in dairy cow.[Method] A total of 70 samples of suspected clinical mastitis cows were detected on gene level by multiple PCR.[Result]A total of 58 samples were reported to be positive with the established multiple PCR method and the positive rate was up to 82.5%.While 53 samples were reported to be positive by the traditional method and the positive rate was up to 75.5%.That identity of the positive rate between traditional method and multiple PCR was 92%.[Conclusion]The multiple PCR method established is rapid and specific for the detection of pathogenic subclinical mastitis in dairy cow.

关 键 词：奶牛隐性乳房炎 多重PCR 检测 

分 类 号：Q93[生物学—微生物学]