蒙药苘麻子中多糖的提取纯化及含量测定  

Determination and Purification of Polysaccharide from Qingmazi

作　　者：赵玉英[1] 司志鑫[1] 刘飞[1] 李敬朋[1] 青松[1] 陈伟[1] 

机构地区：[1]内蒙古民族大学分析测试中心

出　　处：《光谱实验室》2010年第4期1466-1468,共3页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金资助(编号:20661002);内蒙古自然科学基金资助(编号:200711020207)

摘　　要：采用热水提取-乙醇沉淀法从苘麻子中提取多糖,Sevag法除蛋白,苘麻子多糖经大孔树脂分离、透析得两种多糖,即QMZP-1和QMZP-2,采用分光光度法(λ=486nm)测定两种多糖含量,分别为1.8%和0.75%,求得校准曲线的回归方程为y=0.00435x+0.561,相关系数为r=0.9998,平均回收率为100.1%,RSD=0.71%(n=5)。Polysaccharide was extracted from qingmazi with hot water-ethanol deposit method and wiped off deproteination with Sevag method. Polysaccharide of qingmazi was further purified by resin obtained QMZP-1,QMZP-2,and its content was determined by spectrophotometry（λ=486nm）.The regression equation is y=0.00435x＋0.561 and coefficient correlation is 0.9998.The average recovery was 100.1%,RSD=0.71%（n=5）.

关 键 词：多糖 苘麻子 纯化 

分 类 号：O657.32[理学—分析化学]