一个水稻动态窄叶突变体的鉴定和基因定位  

Identification and gene mapping of a rice dynamic narrow leaf mutant

作　　者：曾生元[1] 郭旻[1] 李敏[1] 孟庆彩[1] 胡群[1] 龚志云[1] 顾铭洪[1] 严长杰[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室,教育部植物功能基因组学重点实验室,扬州225009

出　　处：《科学通报》2010年第21期2106-2111,共6页Chinese Science Bulletin

基　　金：农业部转基因专项(2008ZX08009-003);江苏省高校自然科学重大基础研究项目(08KJA210002)资助

摘　　要：叶片形态是作物的重要性状,阐明控制作物叶片形态的遗传机理有助于在作物育种中性状的改良,提升作物的产量潜力.本研究通过辐射获得一个水稻动态窄叶突变体(暂命名为dynamic narrow leaf1,dnl1).形态鉴定表明,与野生型品种93-11相比,dnl1在苗期叶片显著变窄、变短,而成熟期无显著差异;同时,dnl1抽穗期延迟,株高变矮,籽粒数减少,结实率降低.遗传分析表明窄叶性状受1对隐性基因控制,基因初步定位表明Dnl1位于第1染色体长臂的d15和d20标记之间,遗传距离分别为0.9和2.2cM.进一步利用已经公布的SSR标记和发展的STS标记将其定位于STS标记M1-Z47和M1-Z42之间,与d17,M1-Z41,M1-Z43及M1-Z49共分离,物理距离为172kb,为克隆Dnl1奠定了基础.Leaf shape is an important trait in crops. Understanding the genetic mechanism underlying leaf shape will enable us to improve plant architecture and consequently crop yields. In the present study,we obtained a dynamic narrow leaf rice mutant by γ-ray radiation,temporarily named dnl1. Morphological analysis indicated that the dnl1 mutant produced shorter and narrower leaves at the seedling stage when compared with those of the wild type; whereas no significant differences were exhibited at maturity. In addition,the dnl1 mutant also showed longer longevity,shorter plant height,lower seed set and decreased grain number. Genetic analysis showed that the dynamic narrow leaf character was controlled by a recessive gene,located in the region between the STS markers d15 and d20 on the long arm of chromosome 1,with a genetic distance of 0.9 cM and 2.2 cM,respectively. Additional SSR and STS markers were employed for the fine-mapping of Dnl1. The results indicated that Dnl1 was assigned to the region between M1-Z47 and M1-Z42,cosegregating with d17,M1-Z41,M1-Z43 and M1-Z49; the physical distance of the region was 172 kb long. The findings provide a solid foundation for the cloning of Dnl1.

关 键 词：水稻(Oryza SATIVA L.) 动态窄叶 基因定位 

分 类 号：S511[农业科学—作物学]