HPLC测定复方利血平氨苯蝶啶片中利血平的含量  

HPLC dtermination of reserpine in compound hypotensive tablets

作　　者：金鹏飞[1] 闫小燕[1] 邝咏梅[1] 邹定[1] 胡欣[1] 孙春华[1] 

机构地区：[1]卫生部北京医院药学部,北京100730

出　　处：《药物分析杂志》2010年第7期1242-1244,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：中央保健科研课题专项资金(B2009B032);卫生行业专项基金子课题(200902008-03)

摘　　要：目的：应用高效液相色谱技术,建立复方利血平氨苯蝶啶片中利血平的含量测定方法。方法：采用Alltima C18色谱柱（250 mm×4.6 mm,5μm）,以含0.04%庚烷磺酸钠的50 mmol·min^-1磷酸二氢铵溶液（磷酸调至pH3.0）-乙腈（48∶52,v/v）为流动相,流速0.5 mL·min^-1,检测波长268 nm。结果：在本方法中,利血平的线性关系良好,r为0.9999;专属性强,利血平和药品中其它成分以及强制降解产物之间都有良好的分离度;精密度和稳定性良好,RSD均小于2%;加样回收率良好,百分回收率在99.88%-101.4%之间。结论：该方法准确、简单、快速,可作为复方利血平氨苯蝶啶片中利血平的含量测定方法。Objective：To establish an HPLC method for the determination of reserpine in compound hypotensive tablets.Methods：An Alltima C18 column（250 mm×4.6 mm,5 μm）was used for the separation,with 50 mmol·L^-1 ammonium dihydrogen phosphate containing 0.04% heptane sulfonic acid sodium salt（adjusted pH to 3.0 with H3PO4）-acetonitrile（48∶52,v/v）as the mobile phase at the flow rate of 0.5 mL·min^-1.The detection wavelength was 268nm.Results：The method showed good linearity with a correlation coefficient（r）of 0.9999;the specificity study showed satisfactory resolution between reserpine,other ingredients and forced degradation products;the precision and stability were satisfactory with all RSDs below 2%;and the spiked recovery ranged from 99.01% to 101.92%.Conclusion：This method is an accurate,fast and simple method for the determination of reserpine in compound hypotensive tablets.

关 键 词：高效液相色谱 复方利血平氨苯蝶啶片 利血平 

分 类 号：R917[医药卫生—药物分析学]