RP-HPLC法测定角花胡颓子中齐墩果酸与熊果酸的含量  

RP-HPLC determination of oleanolic acid and ursolic acid in Elaeagnus gonyanthes Benth

作　　者：张俊清[1] 陈文芝[2] 蒋德锡[2] 张丹[2] 

机构地区：[1]海南医学院药学系,海口571101 [2]四川大学华西药学院,成都610041

出　　处：《药物分析杂志》2010年第7期1305-1307,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家支撑计划项目课题(2007BA︱27B06)

摘　　要：目的：建立同时测定角花胡颓子中齐墩果酸与熊果酸含量的RP-HPLC法。方法：采用Diamonsil C18（250 mm×4.6mm,5μm）,以甲醇-0.5%乙酸铵（85∶15）为流动相,检测波长为206 nm,流速为1.0 mL·min^-1,柱温30℃。结果：齐墩果酸进样量在0.06-0.36μg（r=0.9995）、熊果酸进样量在0.65-3.9μg（r=0.9997）范围内,线性关系良好,齐墩果酸与熊果酸的平均加样回收率分别为99.2%和102.5%,RSD分别为8.4%和6.0%（n=9）,重复性试验的含量的RSD分别为6.9%和4.2%（n=6）。结论：该法简便、快速、准确,可用于角花胡颓子的质量控制。Objective：To establish an RP-HPLC for determination of oleanolic acid and ursolic acid in Elaeagnus gonyanthes Benth.Methods： The analytical column was Diamonsil C18 column（250 mm×4.5 mm,5 μm）.The mobile phase consisted of methanol-0.5% ammonium acetate（85∶15）.The detective wavelength was 206 nm.The flow rate was 1.0 mL·min^-1 and the column temperature was 30 degrees.Results： Oleanolic acid and ursolic acid showed a good linearity in range of 0.06-0.36 μg（r = 0.9995） and 0.65-3.9 μg（r=0.9997）.The average recovery of oleanolic acid was 99.2% with relative standard deviation（RSD） of 8.4% and the ursolic acid was102.5%with RSD of 6.0%（n=9）,the reproducibility test of content was 6.9% and 4.2%（n=6）.Conclusion： This method is simple,quick,accurate and can be used for quality control of Elaeagnus gonyanthes Benth.

关 键 词：角花胡颓子 齐墩果酸 熊果酸 HPLC 含量测定 

分 类 号：R917[医药卫生—药物分析学]