黄河流域田间棉铃虫对转Bt基因棉抗性监测  

Frequency of Bt Resistance Alleles in Helicoverpa armigera Populations from the Yellow River Cotton-farming Region of China

作　　者：张洋[1] 张帅[1] 崔金杰[1] 

机构地区：[1]中国农业科学院棉花研究所,河南安阳455000

出　　处：《棉花学报》2010年第4期297-303,共7页Cotton Science

基　　金："十一五"国家科技支撑计划项目(No.2006BAD08A07-4);转基因生物新品种培育重大专项(No.2008ZX08012-004;No.2008ZX08011-002);公益性行业(农业)科研专项(nyhyzx07-005);公益性行业科研专项经费项目(200803011-2);国家棉花产业技术体系

摘　　要：利用改进的单雌系F1/F2代法,对黄河流域主要棉区河南安阳县、河北威县、山东武城县的棉铃虫种群进行了系统监测。在2007-2009年,连续3年保守地估计了以上3个主要植棉县棉铃虫种群对Cry1Ac的抗性基因频率。结果表明,山东武城棉铃虫种群3年的抗性等位基因频率分别为0.00086、0.00097和0.00052;安阳田间棉铃虫种群2008和2009年抗性等位基因频率分别为0.00090和0.00103;河北威县的田间棉铃虫种群抗性等位基因频率为0。所有家系F1代和F2代的相对平均发育级别均值比较结果显示,山东武城县棉铃虫种群耐受性高于其它2个地区,其次为安阳县种群,威县种群敏感度最高。总之,我国黄河流域这3个主要棉区田间棉铃虫种群对Cry1Ac毒素还没有产生明显的抗性,抗性基因频率仍处于正常水平,但是棉铃虫对Cry1Ac毒素的耐受性有升高趋势,需要进一步加强全国性的监测工作。In 2007 to 2009, we used genetic method ofisofemale lines F1/F2 to detect the frequency of Bt resistance genes in Helicoverpa armigera populations which collected from Anyang County, Henan Province, Weixian County, Hebei Province and Wucheng County, Shandong Province. A conservative estimation was carried out and showed that the resistance gene frequencies to CrylAc in Anyang County population were 0, 0.00090 and 0.00103 in 2007, 2008 and 2009, respectively. While in Wucheng County population, the resistance gene frequencies were 0.00086, 0.00097 and 0.00052 in 2007, 2008 and 2009, respectively. In Weixian County population we didn＇t detect resistance genes in 2007, 2008 or 2009. In Anyang County population, the relative average development rate （RADR） of cotton bollworm larvae in Fl test has increased significantly year by year. In Weixian County population, the RADR in F1 test has no significant change. In Wucheng County population, the RADR in F1 test showed an obvious induced tendency. In general, the field populations ofH. armigera in Northern China have not evolved prominent resistance to CrylAc, and their resistance frequency to CrylAc is at a normal level. But the tolerance of Helicoverpa armigera to CrylAc toxin tended to increase, and the early resistance detection and alarm system should be initiated in China as early as possible.

关 键 词：单雌系F1/F2代法 抗性基因频率 相对平均发育级别 棉铃虫 

分 类 号：S435.622[农业科学—农业昆虫与害虫防治]