“滋水涵木”法对去势雌兔性激素及免疫器官的影响  

The effect of hormones and immune organs weight about Zishuihanmu therapy on ovariectomized female rabbit

作　　者：文乐兮[1] 张延武[2] 魏飞跃[1] 尤昭玲[1] 龙玲[1] 孟琼[1] 

机构地区：[1]湖南中医药大学,长沙410007 [2]河南中医学院,郑州450008

出　　处：《江西中医学院学报》2010年第3期70-72,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：湖南省教育厅资助课题(07C483)

摘　　要：目的:探讨滋水涵木治法对去势雌兔性激素水平及免疫器官重量的影响。方法:36只日本大耳白兔随机分为4组:假手术组、去势模型组、中药治疗组、西药对照组,每组9只。切除双侧卵巢建立家兔卵巢功能衰退模型,采用滋水涵木立法的补肾复方干预,并以结合雌激素倍美力作对照。连续给药12周后,放射免疫法检测血清雌二醇(E2)、卵泡刺激素(FSH)、黄体生成激素(LH)水平;称取胸腺、肾上腺、脾脏重量,称重后与体重相比计算脏器指数。结果:去势组E2水平降低,FSH和LH水平升高,肾上腺、胸腺、和脾脏各指数均下降,与假手术组比较,差异有统计学意义(P<0.05);中药组和对照组E2水平与去势组比较差异有统计学意义(P<0.05);中药组FSH、LH水平降低,与去势组比较,差异有统计学意义(P<0.05),与假手术组比较,差异无统计学意义;中药组、对照组免疫器官脏器指数均有明显增加,与去势组比较,差异有统计学意义(P<0.05)。结论:调整性激素水平、改善免疫功能可能是滋水涵木法防治PMS的机理之一。Objective：To explore The effect of hormones and immune organs weight about Zishuihanmu therapy on on ovariectomized female rabbit. Methods：36 ovariectomized female rabbits were randomly divided into sham-operated group,model group,treatment group and control group,every groups is 9.the model were made by cuting byside ovary. treatment group and control group gavage 1 time daily for 12 weeks. The end of the experiment,E2,FSH,LH were detected using radioimmunoassay method. Weigh thymus gland,adrenal gland,spleen,then compared with the body weight,calculate visceral index. Results：The model group E2 levels is descended;FSH,LH levels is rised,the visceral index is descended,compare to sham-operated group has significant difference（P〈0.05）,treatment group FSH,LH levels is descended,compare to model group has significant difference（P〈0.05）,to sham-operated group has no significant difference;the visceral index of treatment group and control group is rised,compare to model group has significant difference（P〈0.05）.Conclusion：Regulate hormone levels,improve immune function perhaps the mechanism of Zishuihanmu therapy treat menopausal syndrome.

关 键 词：滋水涵木 补肾复方 围绝经期综合征 去势雌兔 性激素 肾上腺 脾脏 胸腺 

分 类 号：R285.5[医药卫生—中药学]