氧化铁磁性纳米颗粒作为MDR1反义寡聚核苷酸载体的构建  

Construction and analysis of MDR1 magnetic nano-antisense probe

作　　者：王金玉[1,2] 武兆忠[1] 李园[1] 

机构地区：[1]广州医学院第二附属医院核医学科,医学影像实验室 [2]广东省珠海市人民医院,519000

出　　处：《实用医学杂志》2010年第13期2270-2273,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金面上项目(编号:30470516)

摘　　要：目的：构建磁性纳米MDR1反义探针，评价以氧化铁磁性纳米颗粒作为载体转染MDR1反义基因的可行性。方法：应用多聚赖氨酸修饰葡聚糖包裹氧化铁纳米颗粒（dextrancoatedironoxidenanopartieles），并将此复合物与肿瘤多药耐药反义基因结合。采用琼脂糖凝胶电泳分析氧化铁磁性纳米颗粒结合MDR1反义基因的能力，结合氧化铁纳米颗粒作为载体进行荧光标记的反义寡聚脱氧核苷酸（asODN）体外细胞转染实验。结果：琼脂糖凝胶电泳显示纳米氧化铁在不同的pH值及各种质量比均有良好的DNA结合能力。纳米氧化铁可作为基因载体将MDR1反义基因转染细胞，荧光显微镜可观察到发绿色荧光的细胞，普鲁士蓝铁染色观察到细胞内的蓝色铁颗粒。结论：成功构建了MDR1反义基因磁性纳米颗粒。纳米氧化铁可作为基因载体转染人细胞。Objective To construct MDR1 antisense magnetic nanopartieles, evaluate the feasibility of using magnetic iron oxide nanoparticles as vectors to transfect MDR1 antisense gene. Methods Dextran coated iron oxide nanoparticles （DCIONP） were modified by cationic polymer Poly-L-Lysine, and the compound was hound to MDR1 antisense gene. The potential of MDR1 antisense gene adsorbed by DCIONP was analyzed by agarose gel electrophoresis. The fluorescent conjugated antisense iron oxide nanopartieles were evaluated as gene carrier in vitro. Results Agarose gel electrophoresis showed that DCIONP had satisfactory potential to adsorb DNA at different pH values and quality ratios. DCIONP could transfer gene into cell. The green fluorescent cells were observed by fluorescence microscope. The dyed blue particles inside the cells were observed by Perl＇s blue iron staining. Conclusions MDR1 antisense magnetic nanopartieies are constructed successfully. DCIONP are elucidated the potential as gene carriers.

关 键 词：氧化铁磁性纳米颗粒 多药耐药 反义寡聚核苷酸 

分 类 号：R346[医药卫生—基础医学]