液泡转化酶反义基因对番茄的遗传转化  

Genetic Transformation of Vacuole Anti-invertase Gene for Tomato

作　　者：梁静娜[1] 程智慧[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《西北植物学报》2010年第7期1316-1319,共4页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30370977);国家"十一五"科技支撑计划(2007BAD79B01)

摘　　要：以中蔬4号番茄的子叶为试材,通过农杆菌介导法遗传转化,将液泡转化酶反义基因导入再生植株,经PCR和Southern斑点杂交检测证明,5株转化植株基因组中整合有目的基因.遗传转化最佳条件为:在附加1.5mg/L6-BA和0.1mg/LIAA的MS培养基上再生培养,外植体预培养2d,菌液浓度OD600=0.5,侵染时间5min,共培养2d.遗传转化后,对整合有目的基因的再生番茄叶片液泡转化酶活性测定,表明液泡转化酶活性明显受到抑制.获得的转基因植株为进一步研究液泡转化酶基因的功能奠定了基础.The cotyledonsegments of tomato（‘Zhongshu No.4’）were infected through Agrobacterium tumefaciens-mediated transformation.The results showed that the vacuolar anti-invertase gene was integrated into the genome of 5 transgenic plants confirmed by PCR and southern dot blotting analysis.The best transformation conditions are as follows：MS medium containing 1.5 mg/L 6-BA and 0.1 mg/L IAA,2 d precultured,infecting for 5 min with OD600 0.5 A.tumefaciens,and co-cultivating for 2 d.The activity of vacuolar invertase was decreased by expression of antisense fragment in tomato leaves.The gene transformed plants lay a foundation for the further research of the function of vacuolar invertase gene.

关 键 词：番茄 不定芽再生 遗传转化 转化酶 反义基因 

分 类 号：Q789[生物学—分子生物学]