高效液相色谱法测定烟草料液中防腐剂  

HPLC Determination of Preservatives in Additive Liquor for Tobacco

作　　者：李海燕[1,2] 肖燕 王淑华 胡秋芬 蔡继宝[1] 

机构地区：[1]中国科学技术大学烟草与健康研究中心,合肥230052 [2]云南省烟草质量监督检测站,昆明650106

出　　处：《理化检验（化学分册）》2010年第7期745-747,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用高效液相色谱法测定烟草料液中防腐剂苯甲酸、山梨酸、对羟基苯甲酸乙酯的含量。样品用10g·L乙酸溶液溶解，经Waters Sep-Pak-C18固相萃取小柱净化。以ZORBAX Stable Bound色谱柱为固定相，2．0g·L^-1磷酸-乙腈（20＋80）混合溶液为流动相，用二极管阵列检测器在250nm处进行测定，三种防腐剂在2．5min内可达到基线分离。方法的回收率为88．0％～101．0％，相对标准偏差（72—7）为3．1％--3．6％。A method of HPLC for the determination of preservatives, including benzoic acid, sorbic acid and ethyl-p-hydroxybenzoate, in additive liquor for tobacco was proposed. The sample was dissolved with 10g·L^-1 HOAc solution, and purified by solid phase extraction with Waters Sep-Pak-Cl8 columrL Three kinds of preservatives were separated on the ZORBAX Stable Bound chromatographic column with a mixture of 2. 0g·L^-1 phosphoric acid-acetonitrile（20＋80） as mobile phase, and determined with the photodiode array detector at 250 nm. Baseline separation of benzoic acid, sorbic acid and ethyl-p-hydroxybenzoate was attained within 2. 5 mira Values of recovery and RSD＇s （n=7） were in the ranges of 88. 0%--101.0% and 3. 1%--3.6% respectively.

关 键 词：高效液相色谱 固相萃取 防腐剂 烟草料液 

分 类 号：O652.63[理学—分析化学]