RP-HPLC法测定益气活血注射液中黄芪甲苷的含量  

\DETERMINATION OF ASTRAGALOSIDE Ⅳ IN YIQIHUOXUE INJECTION BY RP HPLC

作　　者：肖娟[1] 王秀兰[1] 马鹏[1] 庞其捷[1] 

机构地区：[1]华西医科大学制药厂

出　　处：《华西药学杂志》1999年第1期43-44,共2页West China Journal of Pharmaceutical Sciences

摘　　要：采用ＲＰ－ＨＰＬＣ法测定益气活血注射液中黄芪甲苷的含量。用十八烷基硅烷键合相柱，乙腈－水（３１∶６９）为流动相，检测波长２０３ｎｍ。经试验，该法线性关系良好（ｒ＝０．９９９９），回收率为９７．５８％（ｎ＝６），ＲＳＤ为１．１９％。可作为益气活血注射液的含量测定方法。An RP HPLC method was described for the determination of astragaloside Ⅳ in yiqihuoxue injection. The RP HPLC conditions were ODS column, CH 3CN∶H 2O(33∶67) as mobile phase, detection at 203 nm.The average recovery was 97.58%, RSD was 1 19%. The method was sensi tive, rapid and accurate and can be used for the quality control of the injection of yiqihuoxue.

关 键 词：益气活血注射液 黄芪甲苷 RP-HPLC 

分 类 号：R286.0[医药卫生—中药学]